The largest database of trusted experimental protocols

2 protocols using polyethylene glycol 3000 da

1

Synthesis and Characterization of Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gold (III) acetate, iron (III) chloride, sodium oleate, oleic acid 99%, oleylamine, 1,2-hexadecanediol, gallic acid, polyethylene glycol 3000 Da, 1-octadecene, triethylamine, 4-dimethylaminopyridine, Dicyclohexyl Carbodiimide (DCC), 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide (MTT), Phosphate-Buffered Saline (PBS), and ethanol absolute were obtained from Sigma-Aldrich (St. Louis, MO, USA). Hoechst 33342 and Propidium Iodide (PI) were purchased from Merck. Dimethyl Sulfoxide (DMSO), toluene, acetone, hexane, chloroform, dichloromethane, and tetrahydrofuran were supplied by Acros organics. Dulbecco’s Modified Eagle Medium (DMEM), Fetal Bovine Serum (FBS), L-glutamine, and penicillin/streptomycin solution were obtained from Gibco. All reagents were used as received without further purification. Milli-Q water (18.2 MΩ, filtered with filter pore size 0.22 µM) was obtained from Millipore.
+ Open protocol
+ Expand
2

Synthesis and Purification of Functionalized Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents were obtained from commercial suppliers (Sigma Aldrich, Acros Organics, Fisher Scientific, Strem Chemicals and Rapp polymere) were used without further purification. Silver chloride (AgCl, 99.9%), selenium powder (Se, 99.99%), and tri-noctylphosphine (TOP, 97%) were obtained from Strem Chemicals. Lipoic acid, Poly ethylene glycol 3000 Da, Triethylamine, Gold (III) chloride trihydrate (HAuCl4•3H2O, ≥99.9%), tetraoctylammonium bromide (TOAB, 98%), tri-n-octylphosphine oxide (TOPO, 99%), Oleylamine (OLAm, 70%) were purchased from Sigma-Aldrich.
As solvents, Milli-Q water (18.2 MΩ, filtered with a filter pore size of 0.22 μM) from Millipore, toluene, isopropanol, ethanol, acetone, hexane, and anhydrous tetrahydrofuran were used (HPLC grade, Acros Organics). Purification of the water-soluble nanoparticles from free reagents was performed on centrifuge filters PALL with a molecular cut-off of 100 kDa (4000 rpm 20 min).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!