Il 15rαko

All animal experiments were performed in accordance with a protocol approved by the Animal Care and Use Committee of the National Cancer Institute. C57BL/6 WT (Ly5.2⁺) and Ly5.1⁺ congenic mice; mice expressing diphtheria toxin receptor transgene under a CD11c promotor (CD11c-DTR Tg); mice expressing MHC class I-restricted TCR specific for ovalbumin (OT-1 Tg); and mice deficient for Tap1, MHC class II, both CD80 and CD86 B7 molecules, CD28, IL-6, OX40, CD47, and IL-15 receptor alpha (IL-15RαKO) were purchased from Jackson Laboratories. IL-15-deficient (IL-15KO) mice were purchased from Taconic Biosciences. CD11c-DTR Tg mice were crossed with Rag1-deficient mice (Jackson Laboratories) to generate CD11c-DTR-Rag1KO mice. IL-15RαKO and IL-15KO mice were backcrossed to the C57BL/6 strain for 10 additional generations in our facility. IL-15Rα transgenic mice were generated by our group (47 (link)).