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Sf reagent

Manufactured by Nacalai Tesque
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Sourced in Japan

SF reagent is a laboratory chemical used for various analytical procedures. It serves as a core component in specific testing and measurement applications. The detailed technical specifications and intended use of this product are not available at this time.

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Lab products found in correlation

Calcusyn software, by Biosoft (1 mentions) Flat bottomed 96 well plate, by Greiner (1 mentions) I control infinite 200, by Tecan (1 mentions)

2 protocols using sf reagent

1

Cell Viability and Proliferation Assay for Hepatocytes

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Cell viability was assessed using a trypan blue dye exclusion method and cell proliferation was evaluated using a modified methyl-thiazol-diphenyl- tetrazolium (MTT) assay with SF reagent (Nacalai Tesque, Kyoto, Japan) as described previously [37 (link),38 (link)]. Cells, including human primary hepatocytes (Applied Cell Biology Research Institute, Kirkland, WA, USA) were seeded in flat-bottomed 96-well plates (Greiner Labortechnik, Hamburg, Germany) at a density of 1×104 cells in 100 μL medium per well, and incubated with HP-β-CyD at various concentrations for 72 hours. The mean of three replicates was calculated for each concentration. Half-maximal inhibitory concentration values (IC50) were determined by nonlinear regression using CalcuSyn software (Biosoft, Cambridge, UK).
Yokoo M., Kubota Y., Motoyama K., Higashi T., Taniyoshi M., Tokumaru H., Nishiyama R., Tabe Y., Mochinaga S., Sato A., Sueoka-Aragane N., Sueoka E., Arima H., Irie T, & Kimura S. (2015). 2-Hydroxypropyl-β-Cyclodextrin Acts as a Novel Anticancer Agent. PLoS ONE, 10(11), e0141946.
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2

Cell Proliferation Assay with SF Reagent

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Cells (0.8-2 × 10 5 /mL) were seeded according to the respective growing speed. SF reagent (Nacalai Tesque, Kyoto, Japan) was added after 48 h, and the absorbance was measured using the Tecan i-control infinite 200 (Tecan, Männedorf, Switzerland). The relative value was calculated based on the absorbance of the control.
Nakatani K., Matsuo H., Harata Y., Higashitani M., Koyama A., Noura M., Nishinaka-Arai Y., Kamikubo Y, & Adachi S. (2021). Inhibition of CDK4/6 and autophagy synergistically induces apoptosis in t(8;21) acute myeloid leukemia cells. International journal of hematology, 113(2).
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