The substances 4-hydroxytamoxifen (TAM; S7827; Selleck Chemicals LLC, Houston, TX, USA), SRPKIN-1 (HY-116856; MedChemExpress LLC; Monmouth Junction, NJ, USA) and TG003 (HY-15338; MedChemExpress LLC; Monmouth Junction, NJ, USA) were purchased and dissolved in DMSO. SRSF3-targeting siRNA (siSRSF3; sc-38338) and non-targeting siRNA (siCtrl; sc-37007) were obtained from Santa Cruz Biotechnology, Dallas, TX, USA. SRSF5 Human shRNA Plasmid Kit (TL309487; OriGene Technologies, Inc., Rockville, MD, USA) was used. SRSF5 Human Tagged ORF Clone (RC207271) was purchased from OriGene Technologies, Inc., Rockville, MD, USA. pCMV6-Entry Mammalian Expression Vector (PS100001; OriGene Technologies, Inc., Rockville, MD, USA) was used for control overexpression.
Tg003
Manufactured by MedChemExpress
Sourced in United States
TG003 is a chemical compound used in research and development laboratories. It functions as a selective inhibitor of cyclin-dependent kinase 11 (CDK11), which plays a role in cellular processes. The product is intended for in vitro research use only, and its specific applications may vary depending on the research objectives.
Automatically generated - may contain errors
Lab products found in correlation
Gsk3326595, by MedChemExpress (1 mentions)
Srpin340, by Bio-Techne (1 mentions)
Kh cb19, by Bio-Techne (1 mentions)
Thz531, by MedChemExpress (1 mentions)
Osmi 4, by MedChemExpress (1 mentions)
Pladienolide b, by Bio-Techne (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Rwpe 1, by American Type Culture Collection (1 mentions)
2 protocols using tg003
1
Modulation of SRSF3 and SRSF5 in Cells
2
Cell Line Characterization and Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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LNCaP, 22RV1, C4-2, and RWPE-1 cell lines were obtained from the American Tissue Culture Collection (ATCC) and PNT1 cell line was acquired from Sigma. LN95 cell line was kindly provided by Professor Stephen Plymate (University of Washington). LNCaP, 22RV1, C4-2 and PNT1 cells were maintained in RPMI medium supplemented with 10% fetal bovine serum (FBS). RWPE-1 cells were cultured in keratinocyte serum-free media, while LN95 cells were maintained in phenol red-free RPMI supplemented with 10% charcoal-stripped FBS. All cell lines were allowed to adhere for at least one day prior to the treatment with inhibitors. THZ531, OSMI-4, TG003 and GSK3326595 were obtained from MedChemExpress. KH-CB19, Pladienolide B and SRPIN340 were purchased from Tocris.
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