Agilent 6120 quadrupole msd mass spectrometer

To visualize the number and concentration of secondary metabolites in the form of chromatographic peaks, HPLC-UV/vis analysis of actinobacteria crude extracts was accomplished using an Agilent 1260 Infinity HPLC system (Agilent Technologies, Santa Clara, CA, USA) equipped with a Luna (Phenomenex, CA, USA) 5 μm C18 (2) 100 Å, LC column (250 × 4.6 mm; solvent A: 0.1% trifluoroacetic acid (TFA)/water, solvent B: Acetonitrile, flow rate: 1.0 mL min^˗1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. UV-vis inset of full wavelength scan (190–600 nm). The methanolic crude extracts were also analyzed by HPLC-MS via an Agilent 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent 1200 Series quaternary LC system and an Eclipse XDB-CD18 column (5 μm, 150 × 4.6 mm; solvent A: 0.1% formic acid/water, solvent B: 0.1% formic acid/Acetonitrile); flow rate: 0.5 mL min^− 1; 0–30 min, 5–100% B; 30–35 min, 100% B; 35–36 min, 100–5% B; 36–40 min, 5% B; Phenomenex NX-C18 column (250 × 4.6 mm, 5 μm); 254 nm. The molecular masses were obtained for both positive and negative ion mode.

Production and HPLC analysis of TM were conducted according to the method described by Ma et al. (2014b) (link). Liquid chromatography-mass spectrometry (LC-MS) was performed on an Agilent 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, United States) equipped with an Agilent 1290 Series Quaternary LC system and an Eclipse Plus C₁₈ column (100 × 2.1 mm, 1.8 μm).