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Vegfa a 20

Manufactured by Santa Cruz Biotechnology
Sourced in Netherlands

VEGFA (A-20) is a lab reagent produced by Santa Cruz Biotechnology. It is an antibody that recognizes the Vascular Endothelial Growth Factor A (VEGFA) protein. VEGFA is a key regulator of angiogenesis, the process of new blood vessel formation.

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2 protocols using vegfa a 20

1

Western Blot Analysis of Protein Targets

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Cells harvested at different time points were lysed and total protein extracts were used for western blotting using antibodies specific for AR (US Biological, Salem, MA), PSA (Santa Cruz Biotechnology, Dallas, TX), Cbl (C-15) (Santa Cruz Biotechnology, Dallas, TX), TRAF6 (Millipore, Temecula, CA), p27Kip1 (C-19) (Santa Cruz, Biotechnology), IRAK1 (F-4) (Santa Cruz, Biotechnology), ZFAND1 (A-14) (Santa Cruz Biotechnology), FGD4 (Epitomics, Burlingame, CA), ABHD3 (Biorbyt, Cambrige, UK), DOK4 (C-16) (Santa Cruz Biotechnology), EGFR (1005) (Santa Cruz Biotechnology), VEGFA (A-20) (Santa Cruz Biotechnology), α-tubulin (Cell Signaling, Danvers, MA), GAPDH (Sigma-Aldrich, St. Louis, MO). Total extracts (30–50 μg) were directly mixed with Lammeli sample buffer and separated on SDS-PAGE. Immunoblotting was performed using appropriate primary and horseradish peroxidase conjugated respective secondary antibodies. Positive signals were detected using a chemiluminiscence ECL kit (Pierce, Rockford, IL).
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2

Western Blot Analysis of VEGF-A Expression

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Western blot was conducted as previously described with modifications [24 (link)]. The cell lysate was separated under reducing conditions on 12% SDS–polyacrylamide gel and transferred onto polyvinylidene fluoride (PVDF) membranes. After blotting the gel, the PVDF membrane (Carl Roth GmbH, Karlsruhe, Germany) was blocked with 4% skim milk in Tris-buffered saline with 0.1% Tween for 1 h at room temperature. The blot was treated with the first antibodies, against beta-actin (Cell Signaling Technology, Leiden, Netherlands) or VEGF-A (A 20; Santa Cruz Biotechnology, Heidelberg, Germany), overnight at 4 °C in 2% skim milk in Tris buffered saline with 0.1% Tween. After washing, the blot was incubated with anti-rabbit immunoglobulin G (IgG), horseradish peroxide-linked antibody (Cell Signaling Technologies) in 2% skim milk in Tris-buffered saline with 0.1% Tween. Following the final washing, the blot was incubated with Immobilon chemiluminescence reagent (Millipore, Schwalbach, Germany), and the signal was detected with MF-ChemiBis 1.6 (Biostep, Jahnsdorf, Germany). The density of the bands was evaluated using Total laboratory software (Biostep), and the signal was normalized for β-actin.
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