Whole mouse genome 4 44k arrays

Microarray analysis was performed with Agilent Whole Mouse Genome 4 × 44 K arrays. Total RNA was extracted from the samples using TAKARA RNAiso (TAKARA, Dalian, China) according to the manufacturer’s standard operating procedures. The total RNA was quality checked by Agilent Bioanalyzer 2100 (Agilent Technologies, CA) electrophoresis and purified using an RNeasy mini kit (QIAGEN, Dusseldorf, Germany) and RNase-Free DNase Set (QIAGEN). Labeling, hybridization and washing were performed according to the Agilent guidelines. The completed hybridized microarrays were scanned using an Agilent Microarray Scanner (Agilent Technologies, CA). Microarray signals and background information were retrieved using Feature Extraction Software 10.7 (Agilent Technologies) and normalized using GeneSpring Software 12.6.1 (Agilent Technologies). The cutoffs for differential expression were set as an absolute fold-change >2 and a corrected p value <0.05. Transcriptome microarray data are available at the Gene Expression Omnibus under the accession number GSE197223.