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Molecular biology grade reagents and chemicals

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Molecular biology grade reagents and chemicals are a range of high-purity laboratory products designed for use in molecular biology applications. These materials meet stringent quality standards to ensure consistent performance and reliable results in research and diagnostic settings.

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Lab products found in correlation

Mini protean tgxtm gels for western blotting, by Bio-Rad (2 mentions) Alexa fluor 488 or 555 conjugated goat anti rabbit or anti mouse antibodies, by Thermo Fisher Scientific (2 mentions) Sterile culture ware, by Thermo Fisher Scientific (2 mentions) Donkey anti rabbit na934, by GE Healthcare (2 mentions) Thrombin cleavage capture kit, by Merck Group (2 mentions) Ni2 nta chromatography resin, by Merck Group (2 mentions) Superdex 10 300 gl columns for akta fplc, by GE Healthcare (2 mentions) Protein markers for sds page, by Bio-Rad (2 mentions) Amicon spin concentrator, by Merck Group (2 mentions) Plasmid miniprep kit, by Qiagen (2 mentions) Biacore sensor chip nta, by GE Healthcare (2 mentions) Protein assay kit, by Bio-Rad (2 mentions) Rneasy plus universal mini kit, by Qiagen (1 mentions) L sulforaphane sfn, by Merck Group (1 mentions) Rabbit anti nrf2 sc 722, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Molecular biology grade chemicals Molecular biology-grade reagents Molecular biology reagent grade Molecular biology reagents Molecular biology grade Chemical grade reagents Reagent grade chemicals Chemical reagents Reagent grade

4 protocols using molecular biology grade reagents and chemicals

1

Mitoprobe JC-1 Assay Protocol

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Mitoprobe JC-1 kit was purchased from Life Technologies Corporation (Carlsbad, CA); rabbit anti-Slc40al (#NBP1–21502), rabbit anti-Abcb6 (#NBP2–58327), mouse anti-Gstpl (#NB110–60512) and rabbit anti Gsta4 (#H00002941-D01P) were purchased from Novus Biologicals (Littleton, CO). Mini-Protean® TGXTM gels for western blotting were purchased from Bio-rad (Hercules, CA, USA). Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) secondary antibodies were from GE Healthcare (Piscataway, NJ); Alexa Fluor” 488 or 555 conjugated goat anti-rabbit or anti-mouse antibodies were obtained from Invitrogen (Carlsbad, CA). RNeasy Plus Universal Mini Kit (#73404) were purchased from RNAeasy plus mini kit (Qiagen Inc, Germantown, MD). Sterile culture wares were purchased from Fisher Scientific (Pittsburgh, PA, USA) while molecular biology grade reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-rad laboratories (Hercules, CA, USA). RNA Stabilization Reagent (RNA later, cat #76104).
Kaisar M.A., Sivandzade F., Bhalerao A, & Cucullo L. (2018). Conventional and electronic cigarettes dysregulate the expression of iron transporters and detoxifying enzymes at the brain vascular endothelium: In vivo evidence of a gender-specific cellular response to chronic cigarette smoke exposure. Neuroscience letters, 682, 1-9.
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2

Sulforaphane Regulation of Nrf2 and NQO1

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L-Sulforaphane (SFN) was purchased from Sigma-Aldrich (St. Louis, MO). Rabbit anti-Nrf2 (#sc-722) and mouse anti-Nqo1 (#sc-376023) antibodies were from Santa Cruz Biotechnology (Dallas, TX); rabbit anti-Slc40a1 was from Novus Biologicals (Littleton, CO). Mouse anti-Glut1 (clone 5B12.3) from EMD Millipore (Billerica, MA); Mini-Protean® TGXTM gels for western blotting were purchased from Bio-rad (Hercules, CA, USA). Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) secondary antibodies were from GE Healthcare (Piscataway, NJ); Alexa Fluor® 488 or 555 conjugated goat anti-rabbit or anti-mouse antibodies were obtained from Invitrogen (Carlsbad, CA). Sterile culture wares were purchased from Fisher Scientific (Pittsburgh, PA, USA) while molecular biology grade reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-rad laboratories (Hercules, CA, USA).
Sajja R.K., Kaisar M.A., Vijay V., Desai V.G., Prasad S, & Cucullo L. (2018). In Vitro Modulation of Redox and Metabolism Interplay at the Brain Vascular Endothelium: Genomic and Proteomic Profiles of Sulforaphane Activity. Scientific Reports, 8, 12708.
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3

Protein Purification Using Ni2+ Chromatography

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Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in surface plasmon resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
Paudyal S., Alfonso-Prieto M., Carnevale V., Redhu S.K., Klein M.L, & Nicholson A.W. (2015). Combined computational and experimental analysis of a complex of ribonuclease III and the regulatory macrodomain protein, YmdB. Proteins, 83(3), 459-472.
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4

Protein Purification and Characterization

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Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in Surface Plasmon Resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
Paudyal S., Alfonso-Prieto M., Carnevale V., Redhu S.K., Klein M.L, & Nicholson A.W. (2015). Combined computational and experimental analysis of a complex of ribonuclease III and the regulatory macrodomain protein, YmdB. Proteins, 83(3), 459-472.
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