Transpack packaging extract catalog

Lambda in vitro packaging reaction was performed for transgene rescue based on the Transpack instruction manual (Agilent Technologies, Transpack Packaging Extract Catalog #200,220, #200,221, and #200,223). Approximately 10 µL of genomic DNA (200–600 µg/mL) was gently mixed with the Transpack packaging reagent, incubated twice at 30 °C for 1.5 h, and mixed with 700 µL of SM buffer to stop the reaction.

Lambda in vitro packaging reaction was performed for transgene rescue according to the Transpack instruction manual (Agilent Technologies, Transpack Packaging Extract Catalog #200,220, #200,221, and #200,223). Approximately 10 µL of the genomic DNA solution (100—600 μg/mL) was gently mixed with the dedicated Transpack packaging tube and incubated at 30 °C for 1.5 h twice before being mixed with 700 µL of SM buffer that was prepared as follows: 5.84 g NaCl, 2.03 g MgSO₄·7H₂O, 50.0 mL of 1 mol/L Tris–HCl [pH 7.5] (NIPPON GENE Co., Ltd.), and 100 mg gelatin powder (KANTO CHEMICAL Co., Inc.) were mixed in 800 mL of ultrapure water and then autoclaved (121 °C, 20 min), after fixing the 1000 mL volume by ultrapure water.