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2 protocols using 4 nitrophenylhydrazine

1

Synthesis and Characterization of Hybrid Microgels

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Styrene (STY), N-isopropylmethacrylamide (NIPM) (98%), N,N-methylenebisacrylamide (MBIS) (99%), ammonium per sulfate (APs) (99%), sodium dodecyl sulfate (SDS) (98%), sodium borohydride (NaBH4) (98%), 2-nitrophenol (2NP), 4-nitrophenol (4NP), 3-nitrophenol (3NP), 4-chloronitrobenzene (4NCB), 2-nitroaniline (2NA), 3-nitroaniline (3NA), 4-nitroaniline (4NA), 2-amino-4-nitroanisole (2A4NA), 4-nitrophenylhydrazine (4NPH), 2,4-dinitrophenylhydrazine (2,4DNPH), and tetrachloroauric(iii) acid trihydrate (HAuCl4·3H2O) were obtained from Sigma Aldrich (Germany). No further purification of the chemicals was done except STY. STY was purified via vacuum filtration by passing it through alumina (Al2O3) to remove the inhibitor. Deionized water was used in all the experimental work. The membrane for dialysis (cut off = 12 000–14 000) was obtained from Scharlu and was used for the removal of unreacted reactants from the microgel and hybrid microgel dispersions.
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2

Characterization of Isothiocyanate and Hydrazine Compounds

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Chemicals 4-Nitrophenyl isothiocyanate (Sigma-Aldrich), phenyl isothiocyanate (Sigma-Aldrich), 4-nitrophenylhydrazine (BDH) and phenylhydrazine (Scharlau) were used as received. Dimethylsulfoxide-d 6 (99.8 atom% D) from Roth was used as received. Acetonitrile (HPLC grade, Sigma-Aldrich) was distilled over calcium hydride and stored in a glass bottle over 3 Å molecular sieves in a desiccator. Other chemicals were used as received without further purification.
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