The fluorescent dyes 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA), propidium iodide (PI), Fura-2 AM, and JC-1 were purchased from Invitrogen (Carlsbad, CA, USA). The calcium-specific chelator BAPTA-AM was also obtained from Invitrogen. The fatty acids palmitate and oleate, bovine serum albumin (BSA), and low glucose Dulbecco's modified Eagle's medium (DMEM) were purchased from Sigma Aldrich (St. Louis, MO, USA). Primary hepatocytes were cultured on plates coated with Collagen I (Rat Tail) from BD Biosciences (San Jose, CA).
Palmitate and oleate
Manufactured by Merck Group
Sourced in United States
Palmitate and oleate are laboratory chemicals commonly used in various research and scientific applications. Palmitate is the salt or ester of palmitic acid, while oleate is the salt or ester of oleic acid. These compounds are often utilized as components in buffer solutions, cell culture media, and other experimental protocols. Their core function is to provide specific chemical properties and interactions required for the intended experimental or analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Fura 2 am, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Bapta am, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem low glucose, by Merck Group (1 mentions)
Low endotoxin bovine serum albumin, by Merck Group (1 mentions)
2 protocols using palmitate and oleate
1
Primary Hepatocyte Lipid Metabolism Assay
2
Preparation of NEFA Solutions
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Palmitate and oleate (Sigma-Aldrich) were dissolved in ethanol to 100 mM. These NEFA solutions were diluted to 1 mM in a differentiation medium supplemented with 2% (w/v) low endotoxin bovine serum albumin (Sigma-Aldrich) and filtered through a 0.2 µm porosity filter (Sarstedt). The molar ratio of fatty acid to albumin was 3.3.
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