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3 protocols using formaldehyde ch2o

1

Tooth Sectioning and Polishing Protocol

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Tooth storage and mounting utilized ethanol (VWR), formaldehyde (CH2O) (Alfa Aesar), PELCO® liquid silver paint, graphite tape (Ted Pella), and EPO-TEK 301 (Epoxy Technology). Coarse sectioning/polishing utilized CarbiMet SiC grinding paper, Metadi supreme polycrystalline aqueous diamond polishing suspension, Microcloth polishing cloth (Buehler). Enamel sections extracted with focused ion beam techniques were mounted onto Omniprobe lift-out grids, Cu with four posts, prod 460-204 (Ted Pella). A ceria standard, from Standard Reference Material 674b (National Institute of Standards and Technology), or Sigma Aldrich (>99.995% trace metals basis, cat. no 202975), was used for calibration.
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2

Nanomaterial Characterization Protocol

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Unless otherwise specified, all solutions were prepared using ultra-pure water (18.2 MΩ∙cm) dispensed from a Barnstead Nanopure UF+UV unit (Thermo-Fisher Scientific, Waltham, MA). Lactic acid (C3H6O3) (Mallinckrodt Chemicals, Center Valley, PA); propionamide (98%), NaF (Sigma-Aldrich, St. Louis, MO); NaH2PO4, Na2HPO4, HNO3 (65wt%), Mg(NO3)2×6 H2O (99%), Ca(NO3)2×4 H2O (99%, Lot No. 86432), ethanol (VWR, Radnor, PA); Ca(NO3)2×4 H2O (99.98%, Lot No. 61600281), formaldehyde (CH2O) (Alfa Aesar, Tewksbury, MA); (NH4)2HPO4 (99%, Lot A0059707, Merck KGaA, Darmstadt, Germany); PELCO® liquid silver paint, graphite tape (Ted Pella, Redding, CA); EPO-TEK 301 (Epoxy Technology, Billerica, MA); CarbiMet SiC grinding paper, Metadi supreme polycrystalline aqueous diamond polishing suspension, Microcloth polishing cloth (Buehler, Lake Bluff, IL). MM22 microtip coupons for FIB liftout (CAMECA Instruments, Madison, WI).
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3

Nanomaterial Characterization Protocol

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Unless otherwise specified, all solutions were prepared using ultra-pure water (18.2 MΩ∙cm) dispensed from a Barnstead Nanopure UF+UV unit (Thermo-Fisher Scientific, Waltham, MA). Lactic acid (C3H6O3) (Mallinckrodt Chemicals, Center Valley, PA); propionamide (98%), NaF (Sigma-Aldrich, St. Louis, MO); NaH2PO4, Na2HPO4, HNO3 (65wt%), Mg(NO3)2×6 H2O (99%), Ca(NO3)2×4 H2O (99%, Lot No. 86432), ethanol (VWR, Radnor, PA); Ca(NO3)2×4 H2O (99.98%, Lot No. 61600281), formaldehyde (CH2O) (Alfa Aesar, Tewksbury, MA); (NH4)2HPO4 (99%, Lot A0059707, Merck KGaA, Darmstadt, Germany); PELCO® liquid silver paint, graphite tape (Ted Pella, Redding, CA); EPO-TEK 301 (Epoxy Technology, Billerica, MA); CarbiMet SiC grinding paper, Metadi supreme polycrystalline aqueous diamond polishing suspension, Microcloth polishing cloth (Buehler, Lake Bluff, IL). MM22 microtip coupons for FIB liftout (CAMECA Instruments, Madison, WI).
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