Cool snap procf color camera

Slides were evaluated using a Nikon ECLIPSE E600 microscope with a coupled Cool SNAP-Procf Color camera. For each specific organ, 50 images (fields) were randomly acquired at 400x magnification using the software Image Pro version 4.5. After collecting the frames, cells with cytoplasmic HMGB1 were quantified in each of the 50 fields and the median of positive cell number was determined. All analyzes were accomplished in a blind test without prior knowledge of the studied groups. After quantification, frames exhibited in figures were selected as to be more informative according to specific areas in the analyzed tissues. Data were processed using GraphPad prism software v5.1 (La Jolla, USA) using non-parametric statistical tests. Significant differences between groups (controls and DENV fatal cases) were determined using Mann-Whitney test with ***p<0.001.

Slides were evaluated using a Nikon ECLIPSE E600 microscope with a coupled Cool SNAP-Procf Color camera. For each specific antibody, 50 images (fields) were randomly acquired at 400x magnification using the software Image Pro version 4.5. After collecting the frames, positive cells were quantified in each of the 50 fields in every organ and the median of positive cell number was determined. All analyzes were accomplished in a blind test without prior knowledge of the studied groups. After quantification, frames exhibited in figures were selected as to be more informative according to specific areas in the analyzed tissues. S1 Table contains the raw data.