The human cancer cells H23 and A2780, and the HEK-293T epithelial cells were purchased from ATCC (Manassas, USA) and ECACC (Sigma, Spain) and cultured as recommended. The CDDP-resistant variants H23R and A2780R were established in our laboratory as previously described,13 (link),17 using CDDP (Farma Ferrer, Spain) for cell viability assays. To validate the results obtained from the resistant cell lines established in our laboratory, we also used the CDDP-resistant lung cancer cell line H1299, with IC50 over 7, purchased from ATCC and maintained as recommended. Cell authentication is shown in Supplementary Table 3 .
Hek 293t epithelial cells
Manufactured by American Type Culture Collection
Sourced in United States
HEK-293T cells are an immortalized cell line derived from human embryonic kidney cells. They are epithelial-like in morphology and are commonly used in cell and molecular biology research, transfection experiments, and virus production.
Automatically generated - may contain errors
Lab products found in correlation
A2780, by American Type Culture Collection (2 mentions)
H1299, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Huvecs, by Cambrex (1 mentions)
Human tumor necrosis factor α, by Merck Group (1 mentions)
Endothelial growth medium 2, by Thermo Fisher Scientific (1 mentions)
Vcam 1, by Santa Cruz Biotechnology (1 mentions)
Icam 1, by Santa Cruz Biotechnology (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Vero cell, by American Type Culture Collection (1 mentions)
4 protocols using hek 293t epithelial cells
1
Establishing CDDP-Resistant Cancer Cell Lines
2
Establishing CDDP-Resistant Cancer Cell Lines
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The human cancer cells H23 and A2780, and the HEK-293T epithelial cells were purchased from ATCC (Manassas, USA) and ECACC (Sigma, Spain) and cultured as recommended. The CDDP-resistant variants H23R and A2780R were established in our laboratory as previously described,13 (link),17 using CDDP (Farma Ferrer, Spain) for cell viability assays. To validate the results obtained from the resistant cell lines established in our laboratory, we also used the CDDP-resistant lung cancer cell line H1299, with IC50 over 7, purchased from ATCC and maintained as recommended. Cell authentication is shown in Supplementary Table 3 .
3
Inflammatory Cell Interaction Assay
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HUVECs and monocytic U937 cells were obtained from Clonetics (Walkersville, MD, USA), and HEK 293T epithelial cells were obtained from the American Type Culture Collection (#CCL-2, Rockville, MD, USA). Endothelial growth medium-2, Dulbecco’s modified Eagle’s medium, fetal bovine serum, and antibiotics were purchased from Gibco (Grand Island, NY, USA). Human tumor necrosis factor-α (TNF-α) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against cell adhesion molecules (i.e., VCAM-1 and ICAM-1) and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX, USA) and Sigma-Aldrich, respectively. An anti-mouse macrophage specific antibody (galectin-3, clone A3A12) was purchased from Abcam (Cambridge, UK). For analysis with the in vivo imaging system (IVIS), ProSense® 750 FAST Fluorescent Imaging Agent was purchased from PerkinElmer (Santa Clara, CA, USA).
4
Culturing Epithelial Cell Lines
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MDCK epithelial cells, African green monkey kidney epithelial (Vero) cells and human embryonic kidney (HEK 293T) epithelial cells were obtained from the American Type Culture Collection. MDCK cells were maintained in MEM containing 5% heat-inactivated fetal bovine serum (FBS) and 0.5% penicillin–streptomycin. Vero cells were maintained in DMEM (Gibco) containing 10% heat-inactivated FBS and 2 mM L -glutamine. HEK 293T cells were maintained in Opti-MEM (Gibco, NY) supplemented with 10% heat-inactivated FBS. All the cells were maintained at 37 °C in 5% CO2.
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