Chi raa art cellulose sc

The standard of DON, 3-ADON and 15-ADON were purchased from Sigma-Aldrich (St. Louis, MO, USA) and stored at −20 °C prior to use. Acetonitrile, isopropanol, hexane, methanol and ammonium acetate were purchased from Merck (Darmstadt, Germany). In addition, all organic solvents and acids were of analytical or HPLC grade. Milli-Q-quality water (Millipore, Billerica, MA, USA) was used throughout the experiments. The Agilent Extend-C18 (3.0 mm × 150 mm, 3.5 μm) column were obtained from Agilent Technologies Inc (Santa clara, CA, USA). The Waters UPLC BEH amidel (2.1 mm × 100 mm, 1.7 μm) column was purchased from Waters (Milford, MA, USA). The YMC -TRIART diol-HILIC (3.0 mm × 100 mm, 1.9 μm), YMC CHI-RAa ART Cellulose-sc (2.0 mm × 100 mm, 3 μm) and YMC CHIRAa ART Cellulose-sc (3.0 mm × 250 mm, 3 µm) columns were purchased from YMC (Kyoto, Japan).