Collagen matrices were prepared by mixing type I rat tail telocollagen solution and neutralization solution in a ratio of 9:1 at a final concentration of 3.69 mg/ml (lot. 8282, RatCol® Rat Tail Collagen for 3D Hydrogels, Advanced BioMatrix, USA). Type I collagen solution was kept on ice before mixing. After mixing 900 μL of type I collagen solution with 100 μL neutralization buffer, a 1 mL collagen pregel solution was poured into the space between the two glass cylinders, and the inner cylinder was immediately rotated. A 2-minute rotation of the inner cylinder at 50 rpm was applied to generate Couette flow for collagen monomer nucleation on the glass surface and followed by a 20-minute gelling in a stationary condition for the gravitational force to guide collagen fiber elongation. Collagen was polymerized at room temperature.
Ratcol rat tail collagen for 3d hydrogels
Manufactured by Advanced BioMatrix
Sourced in United States
RatCol® Rat Tail Collagen is a purified type I collagen derived from rat tail tendons. It is intended for use in the formulation of 3D hydrogels for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ratcol rat tail collagen for 3d hydrogels
1
Collagen Matrices Fabrication Protocol
2
Engineered Collagen Hydrogel Scaffold
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Collagen hydrogels (10H and 5Ø mm in dimension) were prepared as previously described.45 (link) Briefly, RatCol® Rat Tail Collagen for 3D Hydrogels (Advanced BioMatrix, Carlsbad, CA, USA) and its neutralization solution were mixed in the proportion 9:1 keeping the solution in ice to prevent polymerization. Then cell suspension containing 105 cells was added to a final volume of 300 µL. Hydrogels were let polymerize in 96 well plates for 1 h at 37 °C and then transferred in 2 mL medium/scaffold (well) in the 12-well custom PMMA culture chambers. These were then connected to the bioreactor system operating for up to 7 days, supplying fresh medium three times/week.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!