The largest database of trusted experimental protocols

3 protocols using epitestosterone

1

Quantification of Eicosanoids by LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reference standards of the 32 eicosanoids used in this study (Table 1) were obtained from Cayman Chemical (Ann Arbor, MI, USA). The following internal standards were also purchased from Cayman Chemical: 3,3,4,4-d4-6-keto PGF for 6-keto PGF, 3,3,4,4-d4-PGE2 for the seven prostaglandins and for 11-dehydro TXB2, 3,3,4,4-d4-8-iso PGF for the ten isoprostanes, 6,7,14,15-d4-LTB4 for the four leukotrienes, 5,6,8,9,11, 12,14,15-d8-12(S)-HETE for TXB2 and for the five hydroxyeicosaeonic acids, and 5,6,8,9,11,12,14,15-d8-AA for AA. Epitestosterone was obtained from Steraloids (Newport, RI, USA). For solid-phase extraction (SPE), an Oasis MAX cartridge (1 mL, 30 mg; Waters; Milford, MA, USA) was preconditioned with 1 mL methanol followed by 1 mL deionized water. L-Ascorbic acid (reagent-grade) and formic acid (mass spectrometry-grade, 98% purity) were obtained from Sigma (St. Louis, MO, USA), while HPLC-grade methanol and acetonitrile were acquired from Burdick & Jackson (Muskegon, MI, USA). Deionized water was prepared using a Milli-Q purification system (Millipore; Billerica, MA, USA). Ultrafree-MC polyvinylidene fluoride membrane (0.1-µm pore size) filters were obtained from Millipore.
+ Open protocol
+ Expand
2

Steroid Standards Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lyophilized pure standard E2, E1, 17OHP5, DHT, pregnenolone, progesterone, 17OH-progesterone, androstenedione, testosterone, epitestosterone, dehydroepiandrosterone (DHEA) and cortisol were from Steraloids (Newport, RI, USA); E2-[2,3,4-13 C3] ( 13 C3-E2, isotopic purity >99% 13 C) and estrone-[2,3,4-13 C3] ( 13 C3-E1, isotopic purity >99% 13 C), methanol-[ 2 H4] (D4-methanol, isotopic purity 99.96% D) and bovine serum albumin (BSA) were from Sigma Aldrich (St. Louis, MO, USA). Certified reference standard E2, E1, DHT, 17OHP5 and DHT- [16,16,17-2 H3] (D3-DHT; isotopic purity: 96.15% D3, 0.91% D0/D3) were from Cerilliant (Round Rock, Texas). D3-17OH-pregnenolone (d3-17OHP5, 97% D) was from CDN Isotopes (Pointe Claire, Canada). Lichrosolv grade methanol, ethyl-acetate and N-hexane, granular food-grade activated charcoal were from Merck (Darmstadt, Germany). Ultrapure water was produced by MilliQ Gradient A10 system (Millipore, Volketswil, Switzerland) supplied with double-distilled H2O.
+ Open protocol
+ Expand
3

Steroid Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Testosterone, epiTestosterone, androsterone, etiocholanolone, 5α-androstane-3α,17β-diol, 5βandrostane-3α,17β-diol, dehydroepiandrosterone, 5β-androstanedione, 4-androstenedione and Testosterone-d3 were purchased from Steraloids Inc. (Newport, RI, USA); 5α-dihydroTestosterone was provided by LGC Promochem SRL (Milan, Italy). Isopropyl alcohol, methanol, methyl tertbutyl ether (TBME), ethyl acetate, 17α-methylTestosterone, dithioerythritol and N-Methyl-N-
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!