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Takara ldh cytotoxicity detection kit

Manufactured by Takara Bio
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The TaKaRa LDH cytotoxicity detection kit is a laboratory tool used to measure the activity of lactate dehydrogenase (LDH) enzyme in cell culture samples. LDH is released into the cell culture medium upon cell damage or cell death, and the kit provides a colorimetric method to quantify the LDH activity, which can be used as an indicator of cytotoxicity.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (1 mentions) Cytation 5, by Agilent Technologies (1 mentions)

Close spelling variants of this product

LDH Cytotoxicity Detection Kit (282 citations) Takara kit (48 citations) Cytotoxicity detection kit (21 citations) LDH cytotoxicity kit (10 citations) LDH Detection Kit (10 citations) LDH kit (9 citations)

2 protocols using takara ldh cytotoxicity detection kit

1

Quantifying LDH Cytotoxicity in Cell Cultures

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LDH release into the cell culture supernatant was quantified using a TaKaRa LDH cytotoxicity detection kit (Clontech) and expressed as a percentage of total cellular LDH (100% lysis).
Demarco B., Grayczyk J.P., Bjanes E., Le Roy D., Tonnus W., Assenmacher C.A., Radaelli E., Fettrelet T., Mack V., Linkermann A., Roger T., Brodsky I.E., Chen K.W, & Broz P. (2020). Caspase-8–dependent gasdermin D cleavage promotes antimicrobial defense but confers susceptibility to TNF-induced lethality. Science Advances, 6(47), eabc3465.
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2

Quantifying Cell Permeabilization and Lysis

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Cell permeabilization was quantified by measuring propidium iodide (1 µg/ml; Thermo Fisher Scientific) uptake over time using a fluorescent plate reader (Cytation5; Biotek).
Cell lysis was quantified by measuring the amount of intracellular LDH release into the cell culture supernatant (TaKaRa LDH cytotoxicity detection kit; Clontech). Percentage PI uptake and LDH release were calculated relative to 100 % cell lysis in untreated control sample.
Chen K.W., Demarco B., Heilig R., Ramos S., Grayczyk J.P., Assenmacher C., Radaelli E., Joannas L., Henao‐Mejia J., Brodsky I.E., & Brož P. (2021). RIPK1 activates distinct gasdermins in macrophages and neutrophils upon pathogen blockade of innate immune signalling.
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