Cells were subjected to SA-β-gal staining using the Senescent β-galactosidase Staining Kit (Beyotime, #C0602) according to the manufacturer’s protocol. Briefly, cells were washed with PBS and fixed with the fixative solution for 15 min. Then, the cells were incubated with the staining solution overnight at 37 °C without CO2. Finally, green-stained positive cells were photographed and counted from three different locations per well by an inverted microscope.
Senescent β galactosidase staining kit
Manufactured by Beyotime
Sourced in China
The Senescent β-galactosidase Staining Kit is a laboratory tool designed to detect and quantify the level of senescent cells in a given sample. The kit utilizes the increased activity of the enzyme β-galactosidase, which is a known biomarker for cellular senescence. The kit provides the necessary reagents and solutions to perform this analysis, allowing researchers to study cellular aging and related biological processes.
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Lab products found in correlation
2 protocols using senescent β galactosidase staining kit
1
Senescence-Associated β-Galactosidase Assay
2
Senescent Cell Quantification via β-Galactosidase Assay
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SA-β-galactosidase activity was determined using the Senescent β-Galactosidase Staining Kit (Beyotime, Shanghai, China). Primary cells were washed twice with PBS after treatment. The cells were then fixed with 4% paraformaldehyde at room temperature for 15 min. Next, the cells were washed three times with ice-cold PBS and incubated in freshly prepared β-galactosidase staining solution at 37 °C free of CO2 incubator overnight. Stained cells were imaged using an Olympus FV1000 confocal laser microscope to evaluate the ratio of aged cells.
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