Anti α tubulin monoclonal antibody sc 5286

For Western blotting, lysates of GE1 cells were separated by 12% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane. The membrane was then incubated for 3 h with anti-amelotin polyclonal antibody (ab122312; Abcam, Cambridge, UK) and anti-α tubulin monoclonal antibody (sc-5286; Santa Cruz Biotechnology, Paso Robles, CA, USA). Peroxidase-conjugated anti-rabbit and mouse IgG antibodies (Sigma-Aldrich, St. Louis, MO, USA) were used as the secondary antibodies. Immunoreactivities were detected using ECL plus Western Blotting Detection Reagents (GE Healthcare, Pittsburg, PA, USA).

For the Western blotting analysis, lysates of HPL cells were separated in 12% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a Hybond 0.2 µm polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Pittsburgh, PA, USA). The membrane was then incubated for 3 h with anti-FDC-SP polyclonal antibody (PAB20711; Abnoba, Taipei City, Taiwan) and anti-α tubulin monoclonal antibody (sc-5286; Santa Cruz Biotechnology Inc., Paso Robles, CA, USA). Rabbit anti-mouse IgG, H & L chain specific peroxidase conjugate was used for the secondary antibodies (Sigma-Aldrich, St, Louis, MO, USA). Immunoreactivities were detected using Clarity plus Western ECL Blotting Substrate.