Blood lactate was measured using the Lactate Plus lactate meter (NOVA Biomedical) with blood collected from the tail as we have previously described [26 ].
Lactate plus lactate meter
Manufactured by Nova Biomedical
Sourced in United States
The Lactate Plus lactate meter is a portable device designed for the measurement of lactate levels in whole blood samples. It provides a rapid and accurate method for monitoring lactate concentrations, which is a key indicator of various physiological and medical conditions.
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Lab products found in correlation
Onetouch ultra glucometer, by LifeScan (2 mentions)
Precision xtra blood glucose ketone monitoring system, by Abbott (1 mentions)
Exer3 6m, by Columbus Instruments (1 mentions)
Onetouch ultra2 glucometer, by LifeScan (1 mentions)
Enzymatic assay, by Fujifilm (1 mentions)
Ultrasensitive elisa, by Crystal Chem (1 mentions)
Accu chek aviva meter, by Roche (1 mentions)
N 1 naphthyl ethylenediamine dihydrochloride, by Merck Group (1 mentions)
1290 infinity, by Agilent Technologies (1 mentions)
Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions)
14 protocols using lactate plus lactate meter
1
Measuring Blood Lactate Levels
2
Supplement Effects on Blood Lactate
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The subjects ingested the randomly assigned treatment (Supp, Caff, or PL) by simple methodology at the start of each of the 3 testing visits. The subject’s blood lactate, utilizing a Lactate Plus Lactate Meter (Nova Biomedical Waltham, Waltham, MA, USA) was taken after ingestion, which would serve as the baseline measurement for the session. Twenty min after ingestion of the treatment (established from typical pre-workout supplementation studies [24 (link),25 (link)]), the subjects completed a warm up on the cycle ergometer at a self-selected resistance for 5 min. Subjects then performed stretching as needed before starting the testing portion of the visit.
3
Exercise-Induced Blood Lactate Measurement
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Blood lactate following exhaustive exercise was measured by the UTSW Metabolic Phenotyping Core. All mice were familiarized to the treadmills for 2 days prior to the exercise session. On day 1, they experienced a 5-min rest on the treadmill followed by running for 5 min at the speed of 8 m/min and then for 5 min at the speed of 10 m/min. On day 2, they experienced a 5-min rest on the treadmill followed by running for 5 min at the speed of 10 m/min and then for 5 min at the speed of 12 m/min.
On day 3 (5 weeks post-dosing), mice were placed on the treadmill for 5 min at rest, followed by running with a starting speed of 10 m/min for 40 min, next by running at speeds that were increased at the rate of 1 m/min every 10 min until the speed reached 13 m/min, and finally by running at speeds that were increased at the rate of 1 m/min every 5 min until exhaustion. The exhaustion time was noted as the time at which the mice remained on the electric shock grid for 5 continuous seconds, without attempting to resume running. Blood lactate was taken before putting the mice onto the treadmill and immediately when the mice came off the treadmill. A drop of blood was collected from the tail vein, and blood lactate concentration was measured using a Lactate Plus lactate meter (Nova Biomedical, Waltham, MA, USA).
On day 3 (5 weeks post-dosing), mice were placed on the treadmill for 5 min at rest, followed by running with a starting speed of 10 m/min for 40 min, next by running at speeds that were increased at the rate of 1 m/min every 10 min until the speed reached 13 m/min, and finally by running at speeds that were increased at the rate of 1 m/min every 5 min until exhaustion. The exhaustion time was noted as the time at which the mice remained on the electric shock grid for 5 continuous seconds, without attempting to resume running. Blood lactate was taken before putting the mice onto the treadmill and immediately when the mice came off the treadmill. A drop of blood was collected from the tail vein, and blood lactate concentration was measured using a Lactate Plus lactate meter (Nova Biomedical, Waltham, MA, USA).
4
Metabolite Quantification in Mice
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Blood glucose and ketone (R β‐hydroxybutyrate) concentration were measured using Precision Xtra™ Blood Glucose & Ketone Monitoring System (Abbott Laboratories, Abbott Park, IL, USA). Blood lactate concentration was measured using Lactate Plus Lactate Meter (Nova Biomedical). IGF‐1 and insulin were relabeled for blinded analysis and subsequently analyzed using Luminex 100 system (Luminex, Austin, TX, USA) with R&D Systems Mouse 1‐Plex Luminex Assay (R&D Systems, Minneapolis, MN, USA) and Milliplex Mouse Multiplex Kit (Millipore, St. Charles, MO, USA), respectively, using manufacturer's protocol.
5
Cardiac Puncture and Lactate Measurement
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Blood was withdrawn from deeply anesthetized animals by cardiac puncture. Lactate levels were measured in plasma using Lactate Plus Lactate Meter (Nova Biomedical).
6
Lactate Export Kinetics in VM-M3 Cells
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VM-M3 cells were seeded for 24 hours on 22-mm 12-well plates in triplicate at a density of 50,000 cells/well. The culture media was then replaced and treatment applied. To determine lactate export, 10uL of treated culture media was aspirated and applied to a lactate detection strip and lactate concentration determined with a LACTATE PLUS Lactate Meter (Nova Biomedical) at time of treatment application and every 12 hours over a period of 48 hours.
7
Cardiac Puncture Blood Sampling for Lactate Analysis
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Blood was drawn from deeply anesthetized animals by cardiac puncture. Lactate levels were measured in plasma using Lactate Plus Lactate Meter (Nova Biomedical).
8
Stepwise Treadmill Exercise Test
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Mice were habituated to a motorized treadmill (Exer3/6M, Columbus Instruments) for three days prior to testing (10 min sessions) and given at least 1 day break between habituation and testing. Exercise tolerance was determined by a stepwise progressive test, during which the mice initially ran at a speed of 7.5 m/min and a 5% incline for 5 min. The speed of the treadmill was then increased incrementally until mice were unable to avoid an electrical shock grid at the back of the treadmill, at which time the electrical stimulus was turned off and running times and distances were recorded. Lactate and glucose were determined pre- and post-exercise using a Lactate Plus lactate meter (Nova Biomedical) and a glucometer (Accu-chek Aviva Plus), respectively. Habituation and exercise tests were performed during the day, between 10 am and 4 pm.
9
Measuring Platelet Lactate Production
10
Treadmill Exhaustion Test in Mice
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For exercise studies shown in Figures 1 , 2 , and 4 , mice were run to exhaustion on a closed treadmill (Columbus Instruments) with a shock grid that delivered a mild electrical stimulus (16–28 V) to encourage continuous running. Food was removed 4.5 h before exercise, bedding was replaced with aspen chip bedding and initial body weights were recorded. Mice were weighed immediately before the run and then acclimated to the treadmill with 0° incline at 0 m/min for 5 min. The speed was then increased to 5 m/min and maintained for 5 min. After 5 min of continuous running speed was increased to 10 m/min, after 10 min speed was increased to 15 m/min, after 20 min speed was increase to 25 m/min and after 35 min the speed was increase to 30 m/min and maintained until the mice reached exhaustion. Exhaustion was determined by refusal of mice to remain on the treadmill belt for 10 seconds and lack of movement upon removal from the treadmill. Blood glucose and lactate were measured with a One-Touch Ultra glucometer (LifeScan) and a Lactate Plus lactate meter (NovaBiomedical), respectively, from a single drop of blood from the tail vein. Blood glucose and lactate concentrations were measured immediately upon exhaustion (T=0), and at T=5, 10, 15, 30, and 60 min post exhaustion. Mice remained fasted during the 1 h post-exercise period.
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