The blood tests results obtained à jeun in all patients were: complete blood count including eosinophils by using the SYSMEX-XN-1000 analyzer and CRP, LDH, Troponin tests by using the COBAS PRO C 503/E 801analyzer. The lab test values were obtained from patient’s files between day 10 and day 15 after the positive SARS-CoV-2 RT-PCR was confirmed or by the direct evaluation of 30 patients with mild forms of COVID-19 disease on the 15th day.
Xn 1000 analyzer
Manufactured by Sysmex
Sourced in Japan, Germany
The XN-1000 analyzer is a compact, fully automated hematology analyzer designed for routine laboratory testing. It performs complete blood count (CBC) and white blood cell differential analysis on patient samples. The XN-1000 utilizes fluorescence flow cytometry technology to provide accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Cobas 6000, by Roche (2 mentions)
Cobas c501 analyzer, by Roche (1 mentions)
Cobas 6000 analyzer, by Hitachi (1 mentions)
Cobas e411, by Roche (1 mentions)
Cobas 4800 analyzer, by Roche (1 mentions)
E501 module analyser, by Roche (1 mentions)
Immulite 2000 il 6, by Siemens (1 mentions)
Unicel dxl 800, by Beckman Coulter (1 mentions)
15 protocols using xn 1000 analyzer
1
Blood Tests in Mild COVID-19
2
Cardiometabolic Risk Assessment Protocol
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Questionnaires were used to collect data on age, gender, education, current smoking, current alcohol use, physical activity, and so on.
After resting for at least 5 mins, the blood pressure of each participant’s left arm was measured with a mercury sphygmomanometer three times, and the mean of the three readings was calculated. Waist circumference was measured at the midpoint between the lower rib edge at the midline and the top of the hip bone.
All blood samples were collected after at least 10 h of fasting overnight. Blood samples collected in ethylenediaminetetraacetic acid (EDTA) anticoagulation tubes were used to analyze blood routine parameters, and blood samples collected in tubes containing inert separation gels were used to analyze the blood biochemistry. All blood samples were transported to the central laboratory of the Third Affiliated Hospital of Southern Medical University within 3 h for analysis. Serum creatinine, fasting blood glucose, high-sensitivity C-reactive protein (hsCRP), serum total cholesterol, serum triglycerides, and serum HDL-C were measured by colorimetry. Whole blood cell counts were assessed using a Sysmex XN-1000 analyzer (Sysmex Corporation, Kobe, Japan). The lymphocyte to HDL-C ratio (LHR) was calculated manually.
After resting for at least 5 mins, the blood pressure of each participant’s left arm was measured with a mercury sphygmomanometer three times, and the mean of the three readings was calculated. Waist circumference was measured at the midpoint between the lower rib edge at the midline and the top of the hip bone.
All blood samples were collected after at least 10 h of fasting overnight. Blood samples collected in ethylenediaminetetraacetic acid (EDTA) anticoagulation tubes were used to analyze blood routine parameters, and blood samples collected in tubes containing inert separation gels were used to analyze the blood biochemistry. All blood samples were transported to the central laboratory of the Third Affiliated Hospital of Southern Medical University within 3 h for analysis. Serum creatinine, fasting blood glucose, high-sensitivity C-reactive protein (hsCRP), serum total cholesterol, serum triglycerides, and serum HDL-C were measured by colorimetry. Whole blood cell counts were assessed using a Sysmex XN-1000 analyzer (Sysmex Corporation, Kobe, Japan). The lymphocyte to HDL-C ratio (LHR) was calculated manually.
3
Differential Blood Counts in Healthy Volunteers
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This study is part of a project approved by the Institutional Ethics Committee of a tertiary care institute in North India, part of which has already been published 12 . This was done on twenty-eight healthy volunteers, after a comprehensive physical and pathological check-up. There were 15 males (aging between 24 years to 41 years) and 13 females (aging between 21 years to 52 years).
The sample collection and analysis procedure for this study has been detailed previously 12 . In brief, samples were collected from all volunteers for six consecutive days between 9.00 AM and 10.00 AM. The analysis was conducted on a quality-controlled Sysmex XN-1000 blood analyzer each day. The Sysmex XN 1000 analyzer estimates the Differential counts by flowcytometry using semiconductor laser after red cell lysis and permeabilization in a dedicated channel separate from the Nucleated red-cell channel, resulting in accurate counts 13, 14 . The analysis started within four hours of collection and the values for NC, LC, EC, MC and BC extracted from the analyzer.
The sample collection and analysis procedure for this study has been detailed previously 12 . In brief, samples were collected from all volunteers for six consecutive days between 9.00 AM and 10.00 AM. The analysis was conducted on a quality-controlled Sysmex XN-1000 blood analyzer each day. The Sysmex XN 1000 analyzer estimates the Differential counts by flowcytometry using semiconductor laser after red cell lysis and permeabilization in a dedicated channel separate from the Nucleated red-cell channel, resulting in accurate counts 13, 14 . The analysis started within four hours of collection and the values for NC, LC, EC, MC and BC extracted from the analyzer.
4
Leukocyte Count and Formula Analysis
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Leukocyte count and formula were carried out on blood samples collected in tubes using EDTA K3 as anticoagulant and processed in a Sysmex XN-1000 analyzer (Sysmex Corporation, Kobe, Japan), which uses flow cytometry fluorescence.
5
Comprehensive Coagulation and Hematological Assessment
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D-dimer levels were determined on ACL Top 700 analyzer (Instrumentation Laboratory, Bedford, MA, USA) using a highly sensitive assay (IL D-dimer HS 500). Prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen were also determined on ACL Top 700 analyzer. Complete blood count was determined on Sysmex XN-1000 analyzer (Sysmex, Kobe, Japan). Serum ferritin levels were determined on Roche Cobas 6000 (Roche Diagnostics, Mannheim, Germany).
6
Comprehensive Blood Analysis for Diagnosis
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Blood samples were taken from all patients on their follow-up prior to their blood transfusion. A total of 6 ml of blood was divided into two ethylenediaminetetraacetic acid (EDTA) tubes. The first tube was analyzed for complete blood count (CBC) using a Sysmex XN 1000 Analyzer (Sysmex Corporation, Kobe, Japan) and run for mini capillary hemoglobin electrophoresis (Sebia 9 Hydragel K20 Hemoglobin; Capillarys® Sebia, Lisses, France), while the other tube for DNA extraction using the QIAamp® DNA Blood Mini Kit lot no. kit. 166051764 (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions. DNA samples were stored at −80°C, before subsequent analysis, i.e., performed polymerase chain reaction (PCR) and followed by the Sanger sequencing on these DNA samples. Ferritin, liver function tests, and renal function tests data were obtained from medical records based on the latest data. Molecular analysis was carried out at the Universitas Gajah Mada (UGM) Integrated Research and Testing Laboratory. Analytical statistics included the calculation of frequency distribution, mean, and standard deviation (SD).
7
Comprehensive Blood Biomarker Profiling
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Nonfasting blood samples were collected in the morning. sTfR serum levels were determined using a commercial particle enhanced immunoturbidimetric assay (Tina-quant Soluble Transferrin Receptor II, Roche Diagnostics, Mannheim, Germany). The assay was performed on a Cobas c 501 analyzer (Roche Diagnostics, Mannheim, Germany). The limit of detection (LOD) of the test was 0.40 mg/l (4.72 nmol/l). The average intra- and interassay coefficient of variation was 1.5 and 1.7%, respectively. Serum ferritin levels were measured using a commercial particle enhanced immunoturbidimetric assay (Tina-quant Ferritin Gen 4, Roche Diagnostics, Mannheim, Germany) performed on a Cobas c 501 analyzer (Roche Diagnostics, Mannheim, Germany). Iron, plasma transferrin concentration, and CRP were measured on a Cobas c 501 analyzer (Roche Diagnostics, Mannheim, Germany; iron: colorimetric assay; transferrin and CRP: immunoturbidimetric assay). The percent plasma transferrin saturation was calculated using the following formula: serum − iron [μg/dl]/serum − transferrin [mg/dl] × 70.9. Blood counts were measured with an automated hematology analyzer (XN1000-analyzer, Sysmex, Norderstedt, Germany). The ESR was determined by Westergren method using an SRS 100/II analyzer (Electa-Lab S.r.l., Forli, Italy).
8
Preparation and Characterization of Citrated Plasma
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Human PPP (3.2% citrated) from healthy donors was bought from the Centre de Transfusion Sanguine des Armées (CTSA). Once obtained, the plasma was stored at −80 °C until aliquoted into 3 mL tubes. Platelets, coagulation factors, and fibrinogen were measured to check plasma quality. A residual platelet count was performed on an XN 1000 analyzer (Sysmex, Kobe, Japan) and was undetectable. Coagulation factors and fibrinogen were measured on a STA-R-Max™ automated coagulation analyzer system (Stago, Asnières-sur-Seine, France) and the results are presented in Table 5 . The plasma was then aliquoted and stored at −80 °C until experimentation. Before each experiment, the plasma was defrosted for 4 min in a 37 °C water bath, vortexed, and then used within 4 h (n = 6).
9
Comprehensive Blood Cell Analysis
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Neutrophil, lymphocyte, monocyte and platelet counts, and MPV were measured within 2 hours after collection of blood samples with the use of Sysmex XN-1000 analyzer. The statistical methods evaluating the data were the chi-square, independent t test and Spearman's Rho correlation.The overall accuracy was determined by the area under the curve, sensitivity and specificity using receiver-operating characteristic (ROC) analysis. In all tests, p<0.05 was considered to be statistically significant.
10
Comprehensive Biochemical and Hematological Analyses
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Biochemical tests (creatinine, creatinine kinase—CK, alaninę aminotransferase—ALT, aspartate aminotransferase—AST, γ-glutamyltransferase—GGT, lactate dehydrogenase—LDH, bilirubin, glucose, cholesterol and triglycerides—TG) were performed on a Cobas 6000 analyzer and a C 501 module (Hitachi, Tokyo, Japan), while hematology tests were conducted on an XN-1000 analyzer (Sysmex Corporation, Singapore), coagulometric tests (INR, fibrinogen and prothrombin time—PT) on an ACL TOP 300 CTS analyzer (Instrumentation Laboratory, Werfen Company, Bedford, MA, USA), and blood gas and co-oximetry on an ABL 90 FLEX PLUS analyzer (Radiometer Medical ApS, Brønshøj, Denmark). IL-6 was determined by electrochemiluminescence assay on COBAS e-411(Roche Diagnostics International Ltd., Rotkreuz, Switzerland).
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