Valsartan was purchased from Tecoland Corporation, (Irvine, CA, USA). Soy phosphatidylcholine (SPC), distearoyl phosphatidylcholine (DSPC), hydrogenated Soy phosphatidylcholine (HSPC), dimyristoylphosphatidylcholine (DMPC), and dimyristoyl phosphatidylglycerol sodium (DMPG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA); cholesterol was purchased from Sigma-Aldrich, (St. Louis, MO, USA). Capmul MCM was purchased from Abitech Corporation (Janesville, WI, USA). Labrafil M 2125 was purchased from Alfa Chemicals (Binfield, Berkshire, UK). Tween 80 was purchased from EMD (Billerica, Massachussetts, USA). Avicel PH102 was purchased from FMC BioPolymers (Philadelphia, PA USA). Cell culture media were purchased from ATC Collection (Manassas, VA, USA); Transwell® plates (6-well) were purchased from Corning Life Sciences (Tewksbury, MA, USA). Cannulated Sprague-Dawley (SD) rats were purchased from Harlan Laboratories (Indianapolis, IN, USA); blank rat plasma was obtained from Biomedical (Winchester, VA, USA). Hard gelatin capsules (size 1) were purchased from Capsugel Inc., Morristown, NJ, USA, and all other reagents used are of analytical grade.
Dimyristoylphosphatidylcholine
Manufactured by Avanti Polar Lipids
Sourced in United States
Dimyristoylphosphatidylcholine is a synthetic phospholipid compound commonly used in laboratory research. It serves as a model lipid system for the study of membrane structure and function.
Automatically generated - may contain errors
Lab products found in correlation
Cholesterol, by Merck Group (4 mentions)
Distearoylphosphatidylcholine, by Avanti Polar Lipids (3 mentions)
1 palmitoyl 2 oleoylphosphatidylcholine, by Avanti Polar Lipids (2 mentions)
Polylysine, by Merck Group (2 mentions)
Cellrox deep red reagent, by Thermo Fisher Scientific (2 mentions)
Dapi fluoromount, by Southern Biotech (2 mentions)
N dodecane, by Merck Group (2 mentions)
Inertsil ods 3, by GL Sciences (2 mentions)
Ab serum, by Merck Group (2 mentions)
Efavirenz, by Avantor (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (2 mentions)
Celltiter 96 aqueous one solution reagent, by Promega (2 mentions)
Bca protein assay, by Thermo Fisher Scientific (2 mentions)
Soy phosphatidylcholine, by Avanti Polar Lipids (1 mentions)
Hydrogenated soy phosphatidylcholine, by Avanti Polar Lipids (1 mentions)
19 hydroxycholesterol, by Steraloids (1 mentions)
Hiload 16 600 superdex 200 column, by Cytiva (1 mentions)
Msp1e3d1, by Addgene (1 mentions)
High affinity ni charged resin, by GenScript (1 mentions)
15 protocols using dimyristoylphosphatidylcholine
1
Valsartan Lipid Nanoparticle Formulation
2
Comprehensive Lipid Extraction and Quantitation
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Approximately 4–5 × 106 MPMs were subjected to monophasic lipid extraction in methanol:chloroform:water (2:1:0.74, v:v:v) as previously described [46 (link),79 (link)]. Pelleted cellular proteins were subsequently dissolved in 0.1 N NaOH to determine the total amount of protein per sample group and used for normalization of lipids detected. Di-myristoyl phosphatidylcholine (Avanti Polar Lipids, Alabaster, AL, USA) and 19-hydroxycholesterol (Steraloids, Newport, RI, USA) were added to samples during lipid extraction at 1 nmol/mg protein as an internal standard for relative quantitation of lipids and sterols. Dried lipid extracts were desalted by washing as previously described and resuspended using 200 µL/mg protein in a solution of methanol containing 0.01% butylated hydroxytoluene [79 (link)]. The samples were stored under a blanket of nitrogen at −80 °C until further analysis.
3
Overexpression of MSP1E3D1 in E. coli
4
Lipid Vesicle Encapsulation of Dual-Labeled DNA
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We encapsulated two kinds of TA-rich DNAs, one labeled with Cy3 and the other labeled with Cy5/Cy7 at both ends, in lipid vesicles as described earlier (6 (link)). Briefly, lipid films were prepared by mixing 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(Cap Biotinyl) (0.05 mg/ml) with dimyristoyl phosphatidylcholine (5 mg/ml) (Avanti Polar Lipids) in chloroform and gently drying with nitrogen gas in glass bottle. The lipid film was hydrated with 100 μl buffer solution containing 100 mM NaCl and 25 mM Tris–HCl pH 8.0, followed by repeated freeze-thaw cycles to homogenize the suspension. Two kinds of DNAs were added to make total volume of 200 μl and concentration of 400 nM for each, which corresponds to one molecule in a spherical volume of 200 nm diameter. The solution was extruded 11 times through membrane filter with 200 nm track-etched pores (Whatman, 800281) to create uniformly sized vesicles. The encapsulation efficiency of 15 ± 3% was estimated as the ratio of the number of vesicles containing two dyes (two Cy3’s, two Cy5’s, or one of each Cy3 and Cy5) to the number of vesicles containing one dye (Cy3 or Cy5).
5
Recombinant FVIII Characterization
6
Reconstitution of Lipid Nanodiscs
7
Lipid Synthesis and Antioxidant Analysis
8
Preparing Lipid Samples with Cosolvents
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The phospholipids dimyristoylphosphatidylcholine (DMPC) and palmitoyl-oleoyl-phosphatidylcholine (POPC) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Urea and TMAO were obtained from Sigma-Aldrich and NaCl from VWR. The samples were prepared so that the co-solute-lipid mass fraction (of urea, TMAO or NaCl) in the dry samples, C lip cosol = m cosol /(m cosol + m lip ), was fixed while the water content in terms of the number of water molecules per lipid n w = N w /N lip is variable. The samples were mixed together by the use of a mortar and pestle to produce a fine powder, which was then subjected to drying under vacuum with a 3 Å molecular sieve for at least 24 hours. According to earlier reports, 27, 28 this drying procedure removes all water from the lipid sample. The dry samples were then loaded into the measurement device under a stream of dry N 2 .
9
Efavirenz-Loaded Lipid Nanoparticle Formulation
10
Efavirenz-Loaded Lipid Nanoparticle Formulation
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Efavirenz was obtained from VWR, USA. Dimyristoylphosphatidylcholine (DMPC) and distearoyl-phosphatidylcholine (DSPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Phospholipon 90H (PL-90H) was obtained from Lipoid LLC (Newark, NJ, USA). Cholesterol, n-dodecane, Roswell Park Memorial Institute media (RPMI), AB serum, Phorbol 12- myristate 13-acetate (PMA), poly-lysine and 10% fetal bovine serum (FBS) were purchased from Sigma Aldrich (St. Louis, MO, USA). Inertsil, ODS-3 was purchased from GL sciences, Torrance, CA, USA. Paraformaldehyde (PFA) was purchased from EMD Chemicals, MA, USA. DAPI fluoromount was obtained from Southern Biotech, AL, USA. Rhodamine 123 was purchased from Marker Gene Technologies, Oregon, USA. All the organic solvents were HPLC grade and obtained from Fisher Scientific (Pittsburgh, PA, USA). CellTiter 96® Aqueous One Solution Reagent was purchased from Promega, Madison, WI, USA. CellROX® Deep Red Reagent was obtained from (CAT 10422) Molecular Probes, Eugene, OR, USA.
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