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Hepb3

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HepB3 is a cell line derived from human hepatocellular carcinoma. It is a commonly used in vitro model for the study of hepatitis B virus infection and related research.

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Lab products found in correlation

Hepg2, by American Type Culture Collection (4 mentions) Dulbecco s modified essential medium, by Thermo Fisher Scientific (1 mentions) Ab181112, by Abcam (1 mentions) Ab52939, by Abcam (1 mentions) Ab126783, by Abcam (1 mentions) 1640 medium, by Thermo Fisher Scientific (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh d16h11, by Cell Signaling Technology (1 mentions) Klf4 d1f2, by Cell Signaling Technology (1 mentions)

4 protocols using hepb3

1

Investigating NEAT1 Function in HCC Cells

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Two human HCC cell lines, HepG2 and HepB3, were obtained from the American Type Culture Collection (Manassas, VA) and cultured as described previously [24] . After being seeded into a 96-well plate (2.5 × 10 3 cells per well) and incubated at 37°C for 24 h, HCC cells were transfected with a blank control, mock control, scrambled siRNA (5'-GCTTGAATTAACTGCAGGCGTATAT-3'), or NEAT1 siRNA Magnetofection (OZ Bioscences, Marseille, France). Then, we collected samples at 0, 24, 48, 72, and 96 h for
Ling Z.A., Xiong D.D., Meng R.M., Cen J.M., Zhao N., Chen G., Li R.L, & Dang Y.W. (2018). LncRNA NEAT1 Promotes Deterioration of Hepatocellular Carcinoma Based on In Vitro Experiments, Data Mining, and RT-qPCR Analysis. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(2).
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2

STAT4 Expression in HCC Cell Lines

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HepG2 and HepB3 cell lines were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA), cultured in Dulbecco’s Modified Essential Medium (Invitrogen Corp., Grand Island, NY, USA). Fetal bovine serum (10% heat-inactivated, Invitrogen Crop), 2 mM glutamine, and gentamicin were supplied in media. Cells were grown in the environment with 37°C temperature, 5% CO2 and 100% humidity. The expression of STAT4 in human HCC cell lines HepG2 and HepB3 was detected by Western blot (data not shown). HepG2 showed higher STAT4 expression than HepB3 cell line according to the result, so HepG2 cell line was used for further in vitro experiments. All in vitro experiments were repeated three times.
Li J., Liang L., Liu Y., Luo Y., Liang X., Luo D., Feng Z., Dang Y., Yang L, & Chen G. (2016). Clinicopathological significance of STAT4 in hepatocellular carcinoma and its effect on cell growth and apoptosis. OncoTargets and therapy, 9, 1721-1734.
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3

Establishing Human Hepatic Cell Lines

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The human hepatic cell lines HepG2 and HepB3 were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). CDK5-siRNA was obtained from Sangon Biotech (Shanghai, China) [17 (link)].
Zhang R., Lin P., Yang H., He Y., Dang Y.W., Feng Z.B, & Chen G. (2017). Clinical role and biological function of CDK5 in hepatocellular carcinoma: A study based on immunohistochemistry, RNA-seq and in vitro investigation. Oncotarget, 8(65), 108333-108354.
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4

Human Liver Cancer Cell Line Culture

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Human liver cancer cell lines, HepG2, PLC, and HepB3 were obtained from American Type Culture Collection (Manassas, VA, United States). Huh7 was obtained from the Japan Society for the Promotion of Science (Tokyo, Japan). These cells were maintained in 1,640 medium (Gibco, Thermo Fisher, Waltham, MA, United States) supplemented with 10% heat-inactivated fetal bovine serum (Hyclone) and penicillin/streptomycin at 37°C in a humidified atmosphere of 5% CO2.
The reagents used in this study were: Antibodies of anti-GINS1 (ab181112), anti-BMI1 (ab126783), and anti-RAS (ab52939) were purchased from Abcam (Cambridge, United Kingdom). KLF4 (D1F2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; D16H11) were obtained from Cell Signaling Technology (Danvers, MA, United States).
Li S., Wu L., Zhang H., Liu X., Wang Z., Dong B, & Cao G. (2021). GINS1 Induced Sorafenib Resistance by Promoting Cancer Stem Properties in Human Hepatocellular Cancer Cells. Frontiers in Cell and Developmental Biology, 9, 711894.
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