The HK-2 human kidney tubular epithelial cell line was attained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (Gibco, USA) containing 10% fetal bovine serum at 37°C in a cell incubator with 5% CO2. Before treatment with LPS, ASP (Sigma Aldrich, USA) and the WNT5A antagonist Box-5 (Merk Millipore, USA), cells were starved for 24 h in medium containing 0.5% FBS. For the treatment experiments, cells were treated with LPS (10 μg/ml) alone for 24 h or ASP (5 × 10−4 M) 12 h after LPS treatment. Box-5 (200 μM) was added at the same time as ASP.
Hk 2 human kidney tubular epithelial cell line
Manufactured by American Type Culture Collection
Sourced in United States
The HK-2 human kidney tubular epithelial cell line is a well-characterized in vitro model derived from normal human kidney. This cell line retains many of the morphological and functional characteristics of proximal tubular epithelial cells, making it a valuable tool for studying kidney biology and related diseases.
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Lab products found in correlation
Dulbecco s modified eagle s medium nutrient mixture f 12, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Caki 1, by Thermo Fisher Scientific (1 mentions)
Hk 2 cell line, by Thermo Fisher Scientific (1 mentions)
Ksf medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using hk 2 human kidney tubular epithelial cell line
1
Investigating Kidney Cell Response to LPS, ASP, and Box-5
2
Cultivation of Renal Cell Carcinoma Lines
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The ccRCC cell lines ACHN, 786-0 and CAKI-1 were purchased from the Institute of Biochemistry and Cell Biology (Shanghai, China). The HK-2 human kidney tubular epithelial cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). ACHN and CAKI-1 cells were cultured in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum (FBS) and 786-0 cells were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The HK-2 cell line was cultured in KSF medium (Gibco; Thermo Fisher Scientific, Inc.) containing epidermal growth factor (PeproTech, Inc., Rocky Hill, NJ, USA). All cells were cultured at 37°C in a humidified incubator with 5% CO2.
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