The inhibitors used in this study and the anti-HMGA2 antibody (#8179) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal anti-Histone H3 antibody (ab1791), rabbit polyclonal anti-Histone H3 (acetyl K9) antibody (ab10812), mouse monoclonal anti-β-actin antibody (ab8226), horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (ab6721) and goat anti-mouse IgG (ab205719) were all purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal anti-p300 (phosphor S1834) antibody (PA5-64531) was purchased from Thermo Fisher Scienti c (Waltham, MA, USA). Besides, Recombinant Human EGF Protein (236-EG) was purchased from R&D systems (Minneapolis, MN, USA).
Cell lines and cell culture SNU-368, SNU-739, Huh-7 and HepG2 cells were obtained from the Cell Bank of the Chinese Academy of Science (Shanghai, China). Cells were cultured in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% FBS (HyClone, Logan, UT, USA) at 37 °C in a humidi ed incubator containing 5% CO 2 .
Cell lines and cell culture SNU-368, SNU-739, Huh-7 and HepG2 cells were obtained from the Cell Bank of the Chinese Academy of Science (Shanghai, China). Cells were cultured in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% FBS (HyClone, Logan, UT, USA) at 37 °C in a humidi ed incubator containing 5% CO 2 .