Ponicidin was isolated from Isodon adenolomus (Kunming Institute of Botany, Kunming, China) as described previously (2 (link)). The HT29 human colorectal cancer cell line was obtained from the American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma-Aldrich), in a humidified incubator containing 5% CO2 at 37°C. In vitro experiments were conducted in triplicate at 70% cell confluence.
Ht29 human colorectal cancer cell line
Manufactured by American Type Culture Collection
Sourced in United States
The HT29 human colorectal cancer cell line is a well-established in vitro model derived from a primary tumor of the colon. It is a commonly used cell line for research purposes in the field of colorectal cancer.
Automatically generated - may contain errors
Lab products found in correlation
Fetal calf serum (fcs), by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Parp antibody, by Santa Cruz Biotechnology (1 mentions)
β actin antibody, by Fortis Life Sciences (1 mentions)
Streptomycin, by Welgene (1 mentions)
12 o tetradecanoylphorbol 13 acetate tpa, by Merck Group (1 mentions)
Rpmi 1640 medium, by Welgene (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
2 protocols using ht29 human colorectal cancer cell line
1
Ponicidin Inhibition of Colorectal Cancer
2
Colorectal Cancer Cell Line HT-29 Cultivation
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The HT-29 human colorectal cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Roswell Park Memorial Institute medium (RPMI) 1640 medium supplemented with 10% fetal bovine serum (WelGene, Daegu, Korea) and 100 µg/mL streptomycin (WelGene) at 37 °C in a 5% CO2 atmosphere. RPMI was purchased from WelGene, PGA was purchased from Bioleaders (Daejeon, Korea), and 12-O-tetradecanoylphorbol-13-acetate (TPA) was purchased from Sigma (St. Louis, MO, USA). TUNEL and Annexin V assay kits were purchased from Millipore (Darmstadt, Germany). Antibodies against phospho-AMPK (Thr172), phospho-ERK (Thr202/Tyr204), phospho-Akt (Thr308), caspase 3, and AMPK from Cell Signaling Technology (Beverly, MA, USA). COX-2 and iNOS antibodies were purchased from Cayman (Ann Arbor, MI, USA). PARP antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). β-Actin antibody was purchased from Bethyl Laboratories (Montgomery, TX, USA).
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