Il 6 percp

Manufactured by Thermo Fisher Scientific

Sourced in Italy

The IL-6-PerCP is a fluorescently labeled antibody that binds to the interleukin-6 (IL-6) protein. It is designed for use in flow cytometry applications to detect and quantify IL-6 expression in cell samples.

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Lab products found in correlation

Il 21 pe, by Thermo Fisher Scientific (2 mentions) Ifn γ fitc, by BD (2 mentions) Cd45 apc h7, by BD (2 mentions) Cd56 pe cy7, by BD (2 mentions) Il 22 apc, by Thermo Fisher Scientific (2 mentions) Cd8α v500, by BD (2 mentions) Il 17a v450, by BD (2 mentions) T bet pe cy7 clone 4b10, by Thermo Fisher Scientific (2 mentions) Cd8α fitc, by BD (2 mentions) Cd49b pe clone dx5, by BD (2 mentions) Cd19 fitc, by BD (2 mentions) Facsverse, by BD (2 mentions) Cd45 apc cy7, by BD (2 mentions) Tnf α pe, by BD (1 mentions) Ifn γ pe, by BD (1 mentions) Il 17f pe, by BD (1 mentions) F4 80 pacific blue, by BioLegend (1 mentions) Cd56 v450, by BD (1 mentions) Cd19 apc, by BD (1 mentions) Ifn γ pe cy7, by BD (1 mentions)

2 protocols using il 6 percp

Flow Cytometry Analysis of Tumor-Infiltrating Lymphocytes

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Flow cytometry analysis was performed by using a BD FACSVerse (BD Biosciences, Milan, Italy) and the following monoclonal anti-human antibodies: CD45-APC-H7, CD3ɛ-PerCP, CD3ɛ-FITC, CD8α-V500, CD56-V450, CD56-PE-Cy7, CD19-FITC, IFN-γ-FITC, IFN-γ-PE, IL-17A-V450, IL-17F-PE, TNFα-PE (all from BD Pharmingen, Milan, Italy), IL-21-PE, IL-22-APC, IL-6-PerCP, T-bet-PE-Cy7 (clone 4B10), RORγt-APC (clone AFKJS-9), RORγt-PE (clone AFKJS-9) (all from eBioscience) and CD68 (BioLegend, San Diego, CA, USA). TILs isolated from mouse colon tumors were stained with the following antibodies: CD3ɛ-Pacific Blue, CD8α-FITC, CD45-APC-Cy7, CD49b-PE (clone DX5), CD19-APC (all from BD Pharmingen) and F4/80-Pacific Blue (BioLegend). In parallel, cells were stained with the respective control isotype antibodies.

De Simone V., Franzè E., Ronchetti G., Colantoni A., Fantini M.C., Di Fusco D., Sica G.S., Sileri P., MacDonald T.T., Pallone F., Monteleone G, & Stolfi C. (2014). Th17-type cytokines, IL-6 and TNF-α synergistically activate STAT3 and NF-kB to promote colorectal cancer cell growth. Oncogene, 34(27), 3493-3503.

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Comprehensive Immune Profiling of TILs

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Flow cytometry analysis was performed by using a BD FACSVerse (BD Biosciences). Human TILs were stained with the following monoclonal antibodies: CD45-APC-H7, CD3ε-PerCP, CD8α-V500, CD56-PE-Cy7, CD19-FITC, IFN-γ-FITC, IL-17A-V450 (all from BD Pharmingen, Milan, Italy), IL-21-PE, T-bet-PE-Cy7 (clone 4B10), RORγt-APC (clone AFKJS-9), Foxp3-FITC (clone PCH101), IL-22-APC, IL-6 PerCP (both from eBioscience), CD68-APC (Biolegend, San Diego, CA). Mouse LPMCs and TILs were stained with the following monoclonal antibodies: CD45-APC-Cy7, CD4-PerCP, CD8α-FITC, CD49b-PE (clone DX5), FoxP3-APC, IL-17A-PE, IFN-γ-PE-Cy7 (both from BD Pharmingen), perforin-APC, granzyme B-PE-Cy7 (all from eBioscience). In parallel, cells were stained with the respective control isotype antibodies.

De Simone V., Ronchetti G., Franzè E., Colantoni A., Ortenzi A., Fantini M.C., Rizzo A., Sica G.S., Sileri P., Rossi P., MacDonald T.T., Pallone F., Monteleone G, & Stolfi C. (2015). Interleukin-21 sustains inflammatory signals that contribute to sporadic colon tumorigenesis. Oncotarget, 6(12), 9908-9923.

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