Hematological analyses were carried out using Coulter HMX Automated Hematology Analyzer (Beckman Coulter Corporation, Miami, FL, USA). Hemoglobin profiles and fetal hemoglobin (HbF) concentrations were determined using capillary electrophoresis (CE; Minicap system, Sebia, Parc Technologique Leonard de Vinci, France).
Minicap system
Manufactured by Sebia
Sourced in France
The Minicap system is a lab equipment product designed for capillary electrophoresis. It is a compact and automated system that enables the separation and analysis of various biological samples.
Automatically generated - may contain errors
4 protocols using minicap system
1
Automated Hematological Analyses
2
Capillary Electrophoresis for CDT Analysis
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CE was used for detection of CDT—Carbohydrate Deficient Transferrin (asialo-, monosialo- and disialotranferrin) [28 (link)]. The CAPILLARYS System (Sebia) with the CAPILLARYS CDT kit, and the MINICAP system were used. The MINICAP system uses the principle of electrophoresis in a thin capillary in an electrolyte solution. Charged molecules were separated on the basis of their electrophoretic mobility in an alkaline buffer at a defined pH of 8.8 using high-voltage direct current [29 (link)].
3
Capillary Electrophoresis of Transferrin
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The electrophoretic analysis of transferrin was performed on a MINICAP system (Sebia, France), according to the manufacturer’s instructions. The MINICAP CDT reagent kit (Sebia, France) was applied. The MINICAP system uses the principle of capillary electrophoresis (CE) in a free solution. The system performs all sequences automatically to obtain a complete transferrin isoform profile for the quantitative analysis of each fraction. The human serum transferrin isoforms were separated in an alkaline buffer (pH 8.8) into five major fractions according to their sialylation level: asialotransferrin, disialotransferrin, trisialotransferrin, tetrasialotransferrin and pentasialotransferrin.
4
Hematological Analysis Using Automated Techniques
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Hematological analyses were carried out using Coulter HMX Automated Hematology Analyzer (Beckman Coulter Corporation, Brea, CA, USA). Hemoglobin profiles and fetal hemoglobin (HbF) concentration were determined using Capillary Electrophoresis (CE, Minicap system, Sebia, Parc Technologique Leonard de Vinci, Evry Cedex, France), as described.
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