Cells were fixed with 4% formaldehyde after 0, 7, 14, and 21 days of differentiation. The fixed cells were washed, permeabilized, blocked, and incubated with albumin (1:100; Santacruz Biotechnology, Dallas, TX, USA) antibodies overnight at 4 °C. A goat anti-mouse IgG FITC-conjugated secondary antibody (1:100; Santacruz Biotechnology) was used to detect the signals. The nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI) mounting solution (Abcam). The fluorescence signals were detected with a fluoroscopic microscope (AxioObserver Z1; Carl Zeiss, Oberkochen, Germany).
4 6 diamidino 2 phenylindole dapi mounting solution
Manufactured by Abcam
4',6-diamidino-2-phenylindole (DAPI) mounting solution is a fluorescent dye that binds strongly to DNA. It is commonly used in microscopy techniques to stain and visualize cell nuclei.
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2 protocols using 4 6 diamidino 2 phenylindole dapi mounting solution
1
Immunostaining for cellular proteins
2
Immunofluorescence Microscopy Protocol
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Cells were briefly seeded on chamber slides overnight. The plates were fixed in 4% paraformaldehyde and permeabilized in 0.1% Triton X-100. The plates were incubated with primary antibodies overnight at 4°C and then incubated with fluorescein isothiocyanate-labeled or Alexa Fluor® 488-conjugated IgG secondary antibodies (Thermo Fisher Scientific). The cells were mounted on microscope slides with a 4′,6-diamidino-2-phenylindole (DAPI) mounting solution (Abcam). The fluorescent images of the cells were photographed and analyzed using a confocal laser scanning (Carl Zeiss LSM 780, Germany).
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