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Echopac pc software

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EchoPAC PC software is a clinical image and data analysis application developed by GE Healthcare. The software is designed to provide comprehensive tools for the review, analysis, and reporting of echocardiographic examinations.

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Lab products found in correlation

Vivid e9, by GE Healthcare (2 mentions) Vivid e90 system, by GE Healthcare (2 mentions) L8 18i d linear, by GE Healthcare (2 mentions) Vivid 7 ultrasound echograph, by GE Healthcare (1 mentions) Vivid 7 ultrasound instrument, by GE Healthcare (1 mentions) I13l transducer, by GE Healthcare (1 mentions) Vivid 7, by GE Healthcare (1 mentions)

8 protocols using echopac pc software

1

Transthoracic Echocardiography in Rats

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For transthoracic echocardiography, rats were anesthetized with isoflurane 2% in 21% oxygen/compressed air at 1 L/min) and placed on a heated plate to maintain body temperature at 37.5 °C, the chest shaved, and a Vivid 7 ultrasound echograph (GE Healthcare, Buc, France) equipped with a M12L linear probe operating at 14 MHz and fitted out with Echopac PC software (GE Medical Systems) was used [10 (link)].
Messaoudi H., Levesque T., Perzo N., Berg E., Feugray G., Dumesnil A., Brunel V., Guerrot D., Eltchaninoff H., Richard V., Kamel S., Durand E., Bennis Y, & Bellien J. (2023). Subtotal Nephrectomy Associated with a High-Phosphate Diet in Rats Mimics the Development of Calcified Aortic Valve Disease Associated with Chronic Renal Failure. Journal of Clinical Medicine, 12(4), 1539.
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2

Echocardiographic Imaging of Preterm Infants

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Echocardiographic imaging was performed by trained technicians blinded to prematurity status using a GE Vivid E9 ultrasound system, and the images were analyzed offline to measure left ventricular mass index and ejection fraction22, 23 with EchoPac PC software (both GE Healthcare, Horten, Norway). The mean of triplicate measurements were utilized for analysis.
Bertagnolli M., Xie L.F., Paquette K., He Y., Cloutier A., Fernandes R.O., Béland C., Sutherland M.R., Delfrate J., Curnier D., Bigras J., Rivard A., Thébaud B., Luu T.M, & Nuyt A.M. (2018). Endothelial Colony‐Forming Cells in Young Adults Born Preterm: A Novel Link Between Neonatal Complications and Adult Risks for Cardiovascular Disease. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(14), e009720.
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3

Echocardiographic Assessment of Ventricular Remodeling

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Echocardiography was performed under anesthesia (ketamine 35 mg/kg, xylazine 3 mg/kg, i.m.), as described previously [49 (link)]. The chest hair was shaved and the rabbits were positioned in a dorsal position without restraint. Data acquisition was performed using a Vivid E9 ultrasound machine (GE Healthcare, New York, NY, USA), with a 12S-D probe suitable for rodent models. The Minimum Transthoracic Echo dataset (2-dimensional, M-mode, Doppler (PW), and tissue Doppler (TVI) recordings from parasternal long- and short- axis, as well as apical 4 chamber views) were acquired as recommended by the American Society of Echocardiography [50 ]. Additionally, strain analyses were also performed from apical long axis and 4-chamber views (Section 4.5). Recordings were analyzed using EchoPAC PC software (ver. 112, GE Healthcare, New York, NY, USA) by a blinded expert. Weight of the left ventricle was calculated by the equation: LV Mass (g) = 0.8 × (1.04 × [([LVIDd + IVSd + LVPWd]3 − LVIDd3)]) + 0.6. To assess the geometry of ventricular remodeling (concentric or eccentric), relative wall thickness (RWT) was calculated using the formula: RWT = 2 × (LVPWd)/LVIDd, and increased RWT was considered to show concentric hypertrophy, as it is suggested by the American Society of Echocardiography’s Guideline.
Priksz D., Bombicz M., Varga B., Kurucz A., Gesztelyi R., Balla J., Toth A., Papp Z., Szilvassy Z, & Juhasz B. (2018). Upregulation of Myocardial and Vascular Phosphodiesterase 9A in A Model of Atherosclerotic Cardiovascular Disease. International Journal of Molecular Sciences, 19(10), 2882.
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4

Echocardiographic Assessment of Murine Myocardial Infarction

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Transthoracic echocardiography was performed on awake echo-probe conditioned mice before MI and on days 4 and 21 after MI in un-anesthetized mice using a Vivid E90 system (GE Healthcare) equipped with an L8–18i-D linear array transducer (15 MHz frequency). Parasternal short- and long-axis images were acquired at a depth of 10 mm using both M-mode and 2D-mode imaging at 250 and 605 frames per second, respectively. Image analysis was performed off-line using EchoPAC PC software (GE Healthcare, version 113). End-diastolic and end-systolic left ventricular internal diameters (LVIDd and LVIDs, respectively) were measured at the maximal and minimal diameters acquired from the parasternal short-axis images at a level selected for visualization of the papillary muscle. Fractional shortening (FS) was determined by calculating the change in left ventricular internal dimensions between end-diastole and end-systole normalized to end-diastolic dimension (LVIDd-LVIDs)/LVIDd.
King K.R., Aguirre A.D., Ye Y.X., Sun Y., Roh J.D., Ng RP J.r., Kohler R.H., Arlauckas S.P., Iwamoto Y., Savol A., Sadreyev R.I., Kelly M., Fitzgibbons T.P., Fitzgerald K.A., Mitchison T., Libby P., Nahrendorf M, & Weissleder R. (2017). IRF3 and Type I Interferons Fuel a Fatal Response to Myocardial Infarction. Nature medicine, 23(12), 1481-1487.
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5

Quantitative Ultrasound Characterization of Calcium

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For every calcium mass, IB analysis was performed offline with EchoPac PC software (version 110.0.0, GE Medical Systems, Milwaukee, Wi, USA) in a random order and blinded for the calcium amount. In each image the IB value (IBcalcium), in decibels, was measured in an ellipsoidal ROI delineating the calcium spot. The area of this ROI was calculated:
areacalcium=(longestdiameterROI2×shortestdiameterROI2)π
For calibration, the IBagar value was measured in a circle-shaped ROI of 1mm diameter, 10mm above the calcium. The calibrated IB value (cIB) of each image was calculated: cIB = (IBcalcium − IBagar) × areacalcium. The short axis cIB score and the long axis cIB score were obtained by summation of the cIB values of short and long axis images respectively. Finally, the “cIB calcium score” was obtained by the mean of the short and long axis cIB values.
Gillis K., Bala G., Roosens B., Remory I., Hernot S., Droogmans S, & Cosyns B. (2016). Quantification of Calcium Amount in a New Experimental Model: A Comparison between Ultrasound and Computed Tomography. PLoS ONE, 11(2), e0148904.
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6

Echocardiographic Evaluation of Cardiac Function in Mice

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A Vivid 7 ultrasound instrument (GE Healthcare, Port Washington, NY) equipped with an i13L transducer (recommended for cardiac studies in rodents, 5.9‐14.1 MHz) was used in all experiments. Mice were anesthetized with 0.8% to 1.5% isoflurane, and a mid–left ventricular (LV) short‐axis view at the level of the papillary muscles was obtained in B‐mode and recorded in M‐mode. LV diameter, LV posterior, and septal wall thicknesses in systole and diastole as well as time intervals were measured and averaged over 3 cardiac cycles using EchoPAC PC software (GE Healthcare, Port Washington, NY). Heart rate, fractional shortening, ejection fraction, cardiac output, and LV mass were calculated using standard equations for rodents (as recommended by VisualSonics, Bothell, WA). Matched baseline and final echo data were available for 9 mice in the WHC+placebo group, 9 in the WHC+hArg group, 5 in WHC‐eTNAP+placebo group, and 6 in the WHC‐eTNAP+hArg group.
Rodionov R.N., Begmatov H., Jarzebska N., Patel K., Mills M.T., Ghani Z., Khakshour D., Tamboli P., Patel M.N., Abdalla M., Assaf M., Bornstein S.R., Millan J.L., Bode‐Böger S.M., Martens‐Lobenhoffer J., Weiss N, & Savinova O.V. (2019). Homoarginine Supplementation Prevents Left Ventricular Dilatation and Preserves Systolic Function in a Model of Coronary Artery Disease. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(14), e012486.
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7

Echocardiographic Assessment of Murine Myocardial Infarction

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Transthoracic echocardiography was performed on awake echo-probe conditioned mice before MI and on days 4 and 21 after MI in un-anesthetized mice using a Vivid E90 system (GE Healthcare) equipped with an L8–18i-D linear array transducer (15 MHz frequency). Parasternal short- and long-axis images were acquired at a depth of 10 mm using both M-mode and 2D-mode imaging at 250 and 605 frames per second, respectively. Image analysis was performed off-line using EchoPAC PC software (GE Healthcare, version 113). End-diastolic and end-systolic left ventricular internal diameters (LVIDd and LVIDs, respectively) were measured at the maximal and minimal diameters acquired from the parasternal short-axis images at a level selected for visualization of the papillary muscle. Fractional shortening (FS) was determined by calculating the change in left ventricular internal dimensions between end-diastole and end-systole normalized to end-diastolic dimension (LVIDd-LVIDs)/LVIDd.
King K.R., Aguirre A.D., Ye Y.X., Sun Y., Roh J.D., Ng RP J.r., Kohler R.H., Arlauckas S.P., Iwamoto Y., Savol A., Sadreyev R.I., Kelly M., Fitzgibbons T.P., Fitzgerald K.A., Mitchison T., Libby P., Nahrendorf M, & Weissleder R. (2017). IRF3 and Type I Interferons Fuel a Fatal Response to Myocardial Infarction. Nature medicine, 23(12), 1481-1487.
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8

Murine Cardiac Function Evaluation

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Murine cardiac function was assessed at baseline and weekly until the end of the 6-week study using transthoracic echocardiography. As previously described [5 (link),6 (link),8 (link),9 (link)], a 13 MHz linear array ultrasound probe (Vivid 7, version 11.2, GE Medical Systems, Milwaukee, WI, USA) was used to obtain all images. The EchoPAC PC software (Vivid 7, version 11.2, GE Medical Systems, Milwaukee, WI, USA) was used for offline post-processing of all images. Two blinded observers (CRE and DSJ) completed echocardiographic evaluation.
Evaluation of intra- and inter-observer variability of LV cavity dimensions and function was performed for 30 randomly selected images. To assess intra-observer variability, measurements for random images were performed by a single trained observer (DSJ) on two separate days, two weeks apart. Evaluation of inter-observer variability was assessed by two independent trained observers (CRE and DSJ). Variability was defined as the difference between the two independent observations divided by the mean of the observations and expressed as absolute values.
Eekhoudt C.R., Bortoluzzi T., Varghese S.S., Cheung D.Y., Christie S., Eastman S., Mittal I., Austria J.A., Aukema H.M., Ravandi A., Thliveris J., Singal P.K, & Jassal D.S. (2022). Comparing Flaxseed and Perindopril in the Prevention of Doxorubicin and Trastuzumab-Induced Cardiotoxicity in C57Bl/6 Mice. Current Oncology, 29(5), 2941-2953.
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