Neuron2A cells were purchased from Tumor Cell Bank of the Chinese Academy of Medical Science (Beijing, P.R. China). The cells were cultured in the RPMI-1640 medium supplemented with 10% fetal bovine serum (HyClone, Logan, Utah, USA) and 1% penicillin/streptomycin at 37 °C and under 5% CO2. PPAR-γ siRNA (si-PPAR-γ) along with scramble RNA (si-Con) was purchased from GenePharma (Shanghai, P.R. China). Twenty-four-well plates were used to seed Neuron2A cells (2×104 cells/well) that were incubated for 24 hours prior to transfection with PPAR-γ siRNA or scramble RNA (100 nM) utilizing Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) in media without serum.
Pparγ sirna
Manufactured by Thermo Fisher Scientific
Sourced in United States
PPARγ siRNA is a small interfering RNA (siRNA) that targets the expression of the peroxisome proliferator-activated receptor gamma (PPARγ) gene. The core function of this product is to facilitate the knockdown of PPARγ expression in cells, allowing for the study of the role of this transcription factor in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Klotho sirna, by Thermo Fisher Scientific (1 mentions)
Scrambled control sirna, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lipofectamine ltx and plus reagent, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Nf κb p65, by Abcam (1 mentions)
Opti minimum essential medium, by Thermo Fisher Scientific (1 mentions)
Pparγ transcription factor assay kit, by Cayman Chemical (1 mentions)
Pparγ antibody, by Abcam (1 mentions)
Chat elisa kit, by MyBioSource (1 mentions)
Gw9662, by Merck Group (1 mentions)
Aβ1 42, by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Griess reagent, by Merck Group (1 mentions)
Ldh assay kit, by Nanjing Jiancheng (1 mentions)
4 protocols using pparγ sirna
1
PPAR-γ Silencing in Neuron2A Cells
2
siRNA Transfection for PPARγ and Klotho
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PPARγ siRNA, Klotho siRNA and control scrambled siRNA (Invitrogen; Thermo Fisher Scientific, Inc.) were transfected with Lipofectamine RNAiMAX (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. Cells were seeded in 24-well plates at 5×104/well and were grown overnight to reach ~80% confluence. The cells were transfected with 30 pmol siRNA and incubated for 48 h, and used for subsequent experiments after the transfection efficiency was confirmed by western blot analysis.
3
Curcumin Modulates Neuroinflammation in Alzheimer's
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Curcumin, GW9662, Aβ1–42, and Griess reagent were purchased from Sigma. Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), and Opti- Minimum Essential Medium (MEM) were producted by Gibco. PPARγ siRNA was synthesized by Invitrogen. Lipofectamine LTX and Plus Reagent was produced by Invitrogen. Choline acetyltransferase (ChAT), glial fibrillary acidic protein (GFAP), Iba-1, NF-κB p65, IκBα, and PPARγ antibodies were obtained from Abcam. IL-1β, TNF-α, and COX-2 ELISA kits were purchased from R&D Company. A choline/acetylcholine (Ach) assay kit was supplied by Abcam. A ChAT ELISA kit was obtained from MyBioSource Inc. A PPARγ transcription factor assay kit and PPARγ ligand binding domain (human recombinant) were purchased from Cayman Chemical. A co-immunoprecipitation (Co-IP) kit produced by Pierce was used, and LDH assay kit was supplied by Nanjing Jiancheng Bioengineering Institute.
4
Preparation of Molecular Tools for PPAR Signaling Studies
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Expression vectors for PPARs, SUMO-1, SENPs, ubiquitin conjugating enzyme 9 (UBC9), and PPAR response element-thymidine kinase-luciferase (PPRE-TK-Luc) reporter vector (pPPRE-TK-Luc) were prepared as previously described (32 (link)). An expression vector for PGC1α was obtained from Dr. Gang Xu (The Chinese University of Hong Kong, Hong Kong). Reporter vectors for SENP2 were also generated: (−1980)-Luc, (−868)-Luc, and (−157)-Luc (33 (link)). Mutations in the nuclear factor-κB (NF-κB) binding site of (−157)-Luc were generated by substituting GGG (−70 to −68 bp) with CTC. Adenovirus containing the human SENP2 expression construct (Ad-SENP2) was prepared as previously described (32 (link)). All small interfering (si)RNAs were purchased from Dharmacon except for PPARγ siRNA (Invitrogen). Polyclonal antibody against SENP2 was produced using the peptide representing amino acid 317–335 of mouse SENP2 as an epitope (Abclon, Korea).
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