All ssDNA, modified ssDNA, and primers were synthesized by Sangon Biotech (Shanghai China). bovine serum albumin (BSA, Sigma-Aldrich, Japan, Catalogue#: V900933), trypsin (Sigma-Aldrich, Japan, Catalogue#: T2600000), IgG (Sigma-Aldrich, US, Catalogue#: 14506), MTS cell proliferation colorimetric assay Kit (Abcam, UK, Catalogue#: ab197010), cell counting kit 8 (CCK8, Sigma-Aldrich, US, Catalogue#: 96992), BrdU cell proliferation ELISA Kit (Abcam, UK, Catalogue#: ab126556), Doxorubicin (Dox, Sangon Biotech, Shanghai, China, Catologue#: A603456), DMEM (Gibco®, Life Technologies, Carlsbad, CA), fetal bovine serum (FBS, Gibco®, Life Technologies, Carlsbad, CA), bovine serum albumin (BSA, Fisher Scientific), yeast tRNA (Sigma-Aldrich, St. Louis, MO, USA), MTS CellTiter reagent (Promega, USA).
Doxorubicin dox
Manufactured by Sangon
Sourced in China
Doxorubicin (DOX) is a chemotherapeutic agent used in the treatment of various types of cancer. It is a cytotoxic drug that inhibits the growth and proliferation of cancer cells by intercalating with DNA and disrupting the process of DNA replication and transcription. DOX can be used in the treatment of hematological malignancies, solid tumors, and certain types of sarcomas.
Automatically generated - may contain errors
Lab products found in correlation
Yeast trna, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Mts cell proliferation colorimetric assay kit, by Abcam (1 mentions)
Brdu cell proliferation elisa kit, by Abcam (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffer saline (pbs), by Thermo Fisher Scientific (1 mentions)
Singlet oxygen sensor green (sosg), by Thermo Fisher Scientific (1 mentions)
Sulfuric acid, by Sinopharm (1 mentions)
Acetone, by Sinopharm (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Cyclohexane, by Sinopharm (1 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
5 protocols using doxorubicin dox
1
Proliferation and Cytotoxicity Assays
2
Doxorubicin Nanoparticle Synthesis and Evaluation
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Doxorubicin (DOX) was purchased from Sangon Biotech. 1,1,1,2,3,4,4,5,5,5‐decafluoropentane was purchased from Nanjing Chemlin Chemical Industry. Sunflower pollens and oxygen were purchased from Taobao. Acetone, cyclohexane, and Sulfuric acid was purchased from SINOPHARM. Trypsin‐EDTA solution, phosphate buffer saline (PBS), Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin (P/S) were purchased from Gibco. The cell Live‐Dead assay kit and CCK‐8 were purchased from KeyGen Biotech corporation. The Reactive Oxygen Species Assay Kit was provided from Beyotime Biotechnology. The Singlet Oxygen Sensor Green (SOSG) was purchased from Invitrogen. 3T3 cells and 4T1 cells were provided by the Chinese Academy of Sciences. The 7‐week female BALB/c Nude mice were bought from the Model Animal Research Center of Nanjing University.
3
Cell Lysis and Analysis Protocol
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RIPA Cell Lysis Buffer (Beyotime Institute of Biotechnology, Shanghai, China). SuperSignal Chemiluminescent HRP Substrate (Thermo Fisher scientific Inc, Rockford, US). PrimeScript RT reagent kit and SYBR Premix Ex Taq II (TaKaRa, Dalian, China). Matrigel, FITC Annexin V and propidium iodide (BD Biosciences, Bedford, US). Doxorubicin (DOX) (Sangon Biotech, Shanghai, China) with ultra-purified H2O as solvent. Cantharidin (Institute for Drug Control, Shanghai, China) with dimethyl sulfoxide (DMSO) as solvent. Trypsin-EDTA, Lipofectamine™ RNAiMAX and TRIzol reagent (Invitrogen, Rockville, US). CHIR99021 and antibody against β-actin (Sigma-Aldrich, Saint Louis, US). Fetal bovine serum (FBS. Gibco, Grand Island, US). U6 snRNA Real-time RT-PCR Normalization kit (GenePharma, Shanghai, China). Cell cycle detection kit (Key GENBioTECH, Nanjing, China). Antibodies against Bcl-2, cleaved PARP, Cyclin E2, CyclinB1, Myt1, Phospho-cdc2 (Tyr15), Phospho-Histone H3 (Ser10), Phospho-Wee1 (Ser642), Vimentin, DKK3, LEF1, GSK-3β, active β-catenin, total β-catenin and phospho-GSK-3β(Cell Signaling Technology, Danvers, US). Nuclear and Cytoplasmic Extraction Kit (Kangwei Century Company, Beijing, China). Transwell (Corning Incorporated, New York, US). Bicin choninic acid (BCA) Protein Assay Kit (abcam, Cambridge, US).
4
3D Collagen Culture of Bladder Cancer Cells
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Human bladder cancer cell line T24 was purchased from the American Type Culture Collection and maintained in RPMI 1640 complete medium (Gibco, MA, USA) supplemented with 10% fetal calf serum (Gibco, MA, USA), at 37°C in a 5% CO2 atmosphere. 3D collagen culture of cancer cells was performed as previously described. Briefly, collagen I was diluted to 0.6 mg/ml with RPMI 1640 complete medium supplemented with 10% fetal calf serum. Cancer cells (1 × 104 (link)) were added into the 295 μl collagen solution containing 25 μl of 10× PBS and 20 μl of 1 N NaOH. After 2 h of 37°C incubation, the solid clotty collagen (containing tumor cells) was added to RPMI 1640 complete medium supplemented with 10% fetal calf serum for further use. Collagen I and type I collagenase were purchased from Sigma (USA). Integrin α2β1 ligand peptide TFA (TFA) and AKT inhibitor Miransertib (Mir) were purchased from MedChemExpress (USA). Chemotherapeutic doxorubicin (DOX) and mitomycin C (MMC) were purchased from Sangon (China).
5
Cell Culture Techniques for Hepatic Studies
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Hep3B and L02 cells were obtained from the Cell Bank of CAS (Shanghai, China). Cells were grown in DMEM, plus 10% FBS and 1% Penicillin-Streptomycin solution (Gibco). APS powder (Macklin) was dissolved in DMSO, and Doxorubicin (Dox) was obtained from Sangon and dissolved in ddH2O.
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