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Uas ryrrnai

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The UAS-RyRRNAi is a laboratory equipment product designed for the analysis of ryanodine receptor (RyR) RNA interference. It provides the necessary tools and components to facilitate the investigation of RyR gene expression using RNA interference techniques.

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Lab products found in correlation

Nubbin gal4, by BD (1 mentions) Uas tkvca, by BD (1 mentions) Uas cg12004 rnai, by BD (1 mentions) Vglut gal4, by BD (1 mentions)

2 protocols using uas ryrrnai

1

Drosophila Genetic Crosses and Stocks

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Drosophila were raised in a 25 °C incubator with a twelve-hour light cycle unless specified otherwise. Virgins from Gal4 lines were collected twice a day from the bottles. Virgins were crossed with males carrying the indicated UAS line constructs in a ratio of females to males 15:5. The crosses were staged for 4 h to collect the correct aged larvae. The wild-type Oregon-R fly line is a long-standing stock in the Zartman lab acquired from the N. Yakoby lab. The following transgenic stocks were obtained from Bloomington Drosophila Stock Center (BDSC): UAS-RyRRNAi (BDSC#31540), UAS-InsRDN(BDSC#8253), UAS-InsRCA(BDSC#8263), UAS-TkvRNAi (BDSC#31041), UAS-TkvCA(BDSC#36537), Nubbin-Gal4 (BDSC#25754), Engrailed-Gal4 (BDSC#25752), UAS-Myc (BDSC#9674), UAS-mTORRNAi (BDSC#33951), UAS-MycRNAi (BDSC#25783), and UAS-mTORDN (BDSC#7013).
Kumar N., Rangel Ambriz J., Tsai K., Mim M.S., Flores-Flores M., Chen W., Zartman J.J, & Alber M. (2024). Balancing competing effects of tissue growth and cytoskeletal regulation during Drosophila wing disc development. Nature Communications, 15, 2477.
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2

Drosophila Neurogenetic Tools Protocol

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Flies were raised at 25°C, and all experiments were performed at 22°C. Fly stocks were from either the Bloomington Drosophila Stock Center (BDSC) or the Vienna Drosophila RNAi Consortium (VDRC) as follows: Vglut-GAL4 (BDSC # 24635), Df(3L)Exel6087 (BDSC #7566), TmepCri (BDSC #91319), MHC82-GAL4 [56 (link)], UAS-CG12004 RNAi (TRP #57429), UAS-CG12004 RNAi (VDRC #101732), UAS-ITPR RNAi (BDSC #51795), UAS-RyR RNAi (BDSC #28919), UAS-NMNAT (BDSC # 39699), UAS-GcaMP5-T2A-TdTomato (BDSC # 80079) and wild type (Canton S or w1118). TmepEx was made by InDroso Functional Genomics (Rennes, France) by homologous recombination to create a 16-base deletion in the first coding exon of CG12004. UAS-mKate:CG12004 (long form) and UAS-mKate:hTMEM184B (human) were created by cloning into the pBID vector, which was a gift from Brian McCabe (Addgene plasmid # 35190; http://n2t.net/addgene:35190; RRID:Addgene_35190). Injections were done at Bestgene (Chino Hills, CA).
Cho T.S., Beigaitė E., Klein N.E., Sweeney S.T, & Bhattacharya M.R. (2022). The putative Drosophila TMEM184B ortholog Tmep ensures proper locomotion by restraining ectopic firing at the neuromuscular junction. Molecular neurobiology, 59(4), 2605-2619.
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