Integrated total dietary fibre assay kit

Manufactured by Megazyme

Sourced in Ireland

The Integrated Total Dietary Fibre Assay Kit is a laboratory equipment product that provides a comprehensive analysis of total dietary fibre content in food samples. The kit utilizes enzymatic and gravimetric techniques to quantify the insoluble, soluble, and resistant starch fractions of dietary fibre.

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2 protocols using integrated total dietary fibre assay kit

Dietary Fibre Fractionation and Analysis

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Dietary fibre can be divided according to its solubility and molecular mass to: insoluble dietary fibre, high-molecular-mass dietary fibre soluble in water that precipitates in 78% aqueous ethanol (SDFP), and low-molecular-mass dietary fibre soluble in water that remains soluble in 78% aqueous ethanol (SDFS). They were determined with the integrated total dietary fibre assay kit (Megazyme, Bray, Ireland) according to AOAC method 2011.25 (18 ), using Shimadzu HPLC system with MetaCarb 67C column (Agilent Technologies, Santa Clara, CA, USA) and LC solution v. 1.22 SP1 software (Shimadzu, Tokyo, Japan).

Čukelj Mustač N., Voučko B., Novotni D., Drakula S., Gudelj A., Dujmić F, & Ćurić D. (2019). Optimization of High Intensity Ultrasound Treatment of Proso Millet Bran to Improve Physical and Nutritional Quality§. Food Technology and Biotechnology, 57(2), 183-190.

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Comprehensive Phytochemical Analysis of Plants

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Hydrochloric acid, bromocresol green and methyl red indicators, boric acid, Folin–Ciocalteu’s reagent and sodium carbonate were supplied by Kemika (Zagreb, Croatia). Kjeldahl tablets were purchased from CarlRoth (Karsruhe, Germany). An integrated total dietary fibre assay kit was purchased from Megazyme (Wicklow, Ireland). Gallic acid (>97%), rosmarinic acid (97%), caffeic acid (HPLC standard), cryptochlorogenic (>98%), chlorogenic acid (95%), rutin trihydrate (>97%), luteolin (>98%), apigenin (>97%), (S)-6-Methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol was supplied from Panreac (Barcelona, Spain) and sulfuric acid, hexane, ethanol, formic acid and acetonitrile from Carlo Erba (Val de Reuil, France). Acetone was supplied by Gram-mol d.o.o (Zagreb, Croatia). All chemicals used for experimental procedures were of analytical grade or HPLC grade.

Šeremet D., Jokić S., Aladić K., Butorac A., Lovrić M., Tušek A.J., Obranović M., Mandura Jarić A., Vojvodić Cebin A., Carović-Stanko K, & Komes D. (2022). Comprehensive Study of Traditional Plant Ground Ivy (Glechoma hederacea L.) Grown in Croatia in Terms of Nutritional and Bioactive Composition. Foods, 11(5), 658.

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