Phospho stat3 thy705

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-STAT3 (Tyr705) is a lab equipment product that detects the phosphorylation of STAT3 at tyrosine 705. This product is used to study the activation and signaling of the STAT3 transcription factor.

Automatically generated - may contain errors

Lab products found in correlation

Ecl prime detection kit, by GE Healthcare (1 mentions) Nrf2 phospho s40, by Abcam (1 mentions) Minute cytoplasmic and nuclear extraction kit, by Invent Biotechnologies (1 mentions) Keap1, by Cell Signaling Technology (1 mentions) Lamin b1, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Anti flag and anti actin antibodies, by Merck Group (1 mentions)

Close spelling variants of this product

Phospho-STAT3 STAT3

2 protocols using phospho stat3 thy705

Quantitative Analysis of Nrf2, STAT3, and Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The whole-cell and nuclear protein (Minute Cytoplasmic and Nuclear Extraction Kits; Invent Biotechnologies, Inc., Eden Prairie, MN, USA) were separated using 7.5 or 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, and probed with antibodies against phospho-Nrf2 (S40; 1 : 15 000; Abcam), phospho-STAT3 (Thy705; 1 : 500; Cell Signaling Technology), Keap1 (1 : 1000; Cell Signaling Technology), phospho-p62 (1 : 1000; Medical & Biological Laboratories Co., LTD), Lamin B1 (1 : 10 000; Abcam), and β-actin (1 : 10 000; Sigma, St Louis, MO, USA). After overnight incubation at 4 °C, the membranes were washed and incubated with appropriate horseradish peroxidase-conjugated secondary antibodies and visualised using the ECL prime detection kit (GE Healthcare, Buckinghamshire, UK).

Matsuoka Y., Nakayama H., Yoshida R., Hirosue A., Nagata M., Tanaka T., Kawahara K., Sakata J., Arita H., Nakashima H., Shinriki S., Fukuma D., Ogi H., Hiraki A., Shinohara M., Toya R, & Murakami R. (2016). IL-6 controls resistance to radiation by suppressing oxidative stress via the Nrf2-antioxidant pathway in oral squamous cell carcinoma. British Journal of Cancer, 115(10), 1234-1244.

+ Open protocol

+ Expand

Western Blot of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard Western-blot assays were performed as previsouly described [39 (link)]. Anti-human LIF antibody (AF250-NA) was purchased from R&D. Antibodies against phospho-p70S6 kinase (p-p70S6K, Thr-389), total p70S6K, phospho-AKT (Ser-473), phospho-4EBP1 (Thr-37/46), total 4EBP1 and phospho-STAT3 (Thy-705) were purchased from Cell Signaling Technology. Antibodies against LIFR (sc-659), total AKT (sc-1618) and total STAT3 (sc-482) were purchased from Santa Cruz Biotechnology. Anti-gp130 antibody was purchased from Millipore. Anti-actin and anti-FLAG antibodies were purchased from Sigma.

Li X., Yang Q., Yu H., Wu L., Zhao Y., Zhang C., Yue X., Liu Z., Wu H., Haffty B.G., Feng Z, & Hu W. (2014). LIF promotes tumorigenesis and metastasis of breast cancer through the AKT-mTOR pathway. Oncotarget, 5(3), 788-801.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!