E el r2856c

Manufactured by Elabscience

Sourced in China

E-EL-R2856c is a laboratory equipment product from Elabscience. It is a device that is used for cell and tissue culture applications. The core function of this product is to provide a controlled environment for the growth and maintenance of cells and tissues.

Automatically generated - may contain errors

Lab products found in correlation

E el r0012c, by Elabscience (12 mentions) E el r0015c, by Elabscience (11 mentions) Seg886hu, by Cloud-Clone (2 mentions) Seg886ra, by Cloud-Clone (2 mentions) E el h0109c, by Elabscience (2 mentions) E el r0016c, by Elabscience (2 mentions) E el h0149c, by Elabscience (1 mentions) E el h0102c, by Elabscience (1 mentions) Goat anti rabbit 7074, by Cell Signaling Technology (1 mentions) Anti nf κb p65 8242, by Cell Signaling Technology (1 mentions) E el m0037c, by Elabscience (1 mentions) E el m2453c, by Elabscience (1 mentions) Anti β actin 60004 1 ig, by Proteintech (1 mentions) Goat anti mouse 7076, by Cell Signaling Technology (1 mentions) Lipopolysaccharide, by Merck Group (1 mentions) Cell counting kit 8 (cck8), by Beyotime (1 mentions) β actin, by Cell Signaling Technology (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Sb203580, by MedChemExpress (1 mentions) Gsh a006 2 1, by Nanjing Jiancheng (1 mentions)

14 protocols using e el r2856c

ELISA for Rat IL-6 and TNF-α

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IL-6 and TNF-α levels in the serum of rats and cells were measured using a rat IL-6 ELISA kit (E-EL-R0015c; Elabscience, Wuhan, China) and a rat TNF-α ELISA kit (E-EL-R2856c; Elabscience) respectively according to the manufacturer’s instructions.

Ye S., Qi X., Liu Y., Zhuang L, & Gu Z. (2023). RIP1/3-dependent programmed necrosis induces intestinal injury in septic rats: RIP1/3-dependent programmed necrosis induces intestinal injury. Acta Biochimica et Biophysica Sinica, 56(1), 106-113.

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Quantifying CIRP and Inflammatory Factors

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The CIRP concentrations in the human and rat serum were detected using CIRP ELISA kits (SEG886Hu and SEG886Ra, Cloud-Clone Corp.) according to the manufacturer's protocol. The concentration difference of CIRP in the human serum between the two time points was recorded as ΔCIRP. The ELISA kits for interleukin 6 (IL-6, E-EL-H0102c and E-EL-R0015c, Elabscience), IL-1β (E-EL-H0149c and E-EL-R0012c, Elabscience), and tumor necrosis factor alpha (TNF-α, E-EL-H0109c and E-EL-R2856c, Elabscience) were used to detect inflammatory factors in the rat serum and the cell culture medium.

Zheng X., Fan Y., Li J., Ma T., Li Y., Wang Q., Yan Y, & Liu W. (2022). Change in Oxidative Stress and Mitochondrial Dynamics in Response to Elevated Cold-Inducible RNA-Binding Protein in Cardiac Surgery-Associated Acute Kidney Injury. Oxidative Medicine and Cellular Longevity, 2022, 3576892.

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Cornuside-Mediated Neuroprotection: Mechanistic Insights

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The supplier of cornuside (purity ≥ 98%), i.e., C₂₄H₃₀O₁₄ (molecular mass: 542.9), was the Clinical Medicine Research Institute of China-Japan Friendship Hospital. The ELISA (enzyme-linked immunosorbent assay) kits for Aβ_1–40 (E-EL-M3009), Aβ_1–42 (E-EL-M3010), IL-1β (E-EL-M0037c), IL-6 (E-EL-M2453c), and TNF-α (E-EL-M3063) in mice, as well as for IL-1β (E-EL-R0012c), IL-6 (E-EL-R0896c), and TNF-α (E-EL-R2856c) in rats were products of Elabscience Biotechnology (Wuhan, China). Primary antibodies including anti-Tau (phospho Ser396) (ab32057), anti-GFAP (60190-1-Ig), anti-C3 (21337-1-AP), anti-S100A10 (11250-1-AP), anti-iNOS (18985-1-AP), anti-PSD95 (20665-1-AP), anti-Nrf2 (16396-1-AP), anti-Histone-H3 (17168-1-AP), anti-GAPDH (60004-1-Ig), and anti-β-actin (60004-1-Ig) antibodies were provided by Proteintech (Wuhan, China). Anti-Iba1 (019-19741) antibody was obtained from FUJIFILM Wako Pure Chemical Corporation. Anti-SYP (PB0462) was a product of Boster Biological Technology (Wuhan, China). The provider of anti-p-NF-κB p65 (#3033), anti-p-AKT (Ser473) (#4060), anti-AKT (#4691), anti-IκB-α(#4814), and anti-NF-κB p65 (#8242) antibodies was Cell Signaling Technology (Danvers, MA, USA), which was also the provider of both the goat anti-rabbit (#7074) and goat anti-mouse (#7076) secondary antibodies.

Shi J.Z., Zheng X.M., Zhou Y.F., Yun L.Y., Luo D.M., Hao J.J., Liu P.F., Zhang W.K., Xu J.K., Yan Y., Xie X.M., He Y.Y, & Pang X.B. (2022). Cornuside Is a Potential Agent against Alzheimer’s Disease via Orchestration of Reactive Astrocytes. Nutrients, 14(15), 3179.

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Anti-inflammatory Pathway Regulation

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SB203580 (Cat. HY-10256) was purchased from MedChemExpress (Monmouth, NJ, America). Lipopolysaccharide was purchased from Sigma (Cat. L4130, St. Louis, MO, America). Antibodies for MAPK14 (Cat. 8690S) and β-actin (Cat. 4970S) were purchased from Cell Signaling Technology (Danvers, Massachusetts, America). Cell counting kit-8 (Cat. C0038) was purchased from Beyotime Biotechnology (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) detection kit of pro-inflammatory cytokines TNF-α (E-EL-R2856c), interleukin (IL)-6 (E-EL-R0015c), and IL-1β (E-EL-R0012c) were purchased from Elabscience (Wuhan, China). In situ cell death detection kit (Cat. 11684795910) was purchased from Roche (Huntsville, German).

She H., Tan L., Wang Y., Du Y., Zhou Y., Zhang J., Du Y., Guo N., Wu Z., Li Q., Bao D., Mao Q., Hu Y., Liu L, & Li T. (2023). Integrative single-cell RNA sequencing and metabolomics decipher the imbalanced lipid-metabolism in maladaptive immune responses during sepsis. Frontiers in Immunology, 14, 1181697.

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Quantifying Neuroinflammation and Antioxidant Markers

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Frozen brain tissue samples were homogenized in ice-cold PBS. The homogenate was centrifuged at 4 °C, 15000×g for 10 min. Protein quantification was carried out using the BCA Protein Assay Kit. The levels of tumour necrosis factor (TNF)-α (E-EL-R2856c, Elabscience, China) and interleukin (IL)-1β (E-EL-R0012c, Elabscience, China) were detected by ELISA according to the manufacturer's instructions. The activity of glutathione (GSH; A006-2-1, Nanjing Jiancheng Bioengineering Institute, China) and glutathione-peroxidase (GSH-Px; A005-1-2, Nanjing Jiancheng Bioengineering Institute, China) were determined by colorimetric method as per the manufacturer's instructions.

Fu Y., Yuan P., Zeng M., Zhang Q., Hou Y., Gao L., Wei Y., Zheng Y., Feng W, & Zheng X. (2024). Dihydroquercetin regulates HIF-1α/AKT/NR2B signalling to improve impaired brain function in rats with metabolic syndrome. Heliyon, 10(9), e29807.

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Multiplex Protein Detection in Biological Fluids

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Cold‐inducible RNA‐binding protein (SEG886Hu and SEG886Ra, Cloud‐Clone Corp.), mitochondrial transcription factor A (TFAM; SEH050Hu, Cloud‐Clone Corp.), interleukin 6 (IL‐6; E‐EL‐H0109c and E‐EL‐R0015c, Elabscience), interleukin 1 beta (IL‐1β; E‐EL‐R0012c, Elabscience), tumour necrosis factor‐alpha (TNF‐α; E‐EL‐R2856c, Elabscience) and high mobility group protein B1 (HMGB1; E‐EL‐R0505c, Elabscience) ELISA kits were used to detect these molecules in the UW solution, human serum and rat serum.

Liu W., Fan Y., Ding H., Han D., Yan Y., Wu R., Lv Y, & Zheng X. (2021). Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission. Journal of Cellular and Molecular Medicine, 25(24), 11310-11321.

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Quantifying Inflammatory Cytokines by ELISA

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Concentrations of IL-6, IL-1β, and TNF-α were analyzed using commercially available ELISA Kits (IL-6, Elabscience Biotechnology Co., Ltd., Wuhan, China, E-EL-R0015c; IL-1β, Elabscience Biotechnology Co., Ltd., E-EL-R0012c; TNF-α, Elabscience Biotechnology Co., Ltd., E-EL-R2856c, respectively) according to the manufacturer’s protocols. The absorbance of the samples was measured at 450 nm with a FlexStation 3 Multi-Mode Microplate Reader (Molecular Devices). IL-6, IL-1β, and TNF-α were calculated according to the standard curves.

He M., Qian K., Zhang Y., Huang X.F., Deng C., Zhang B., Gao G., Li J., Xie H, & Sun T. (2021). Olanzapine-Induced Activation of Hypothalamic Astrocytes and Toll-Like Receptor-4 Signaling via Endoplasmic Reticulum Stress Were Related to Olanzapine-Induced Weight Gain. Frontiers in Neuroscience, 14, 589650.

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Cytokine Profiling in Hippocampus

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After modeling (9 days after surgery), the whole blood and hippocampus tissues were harvested. After 2 h of standing, the whole blood was centrifuged for 3 min at 9,000 rpm. The serum was then collected to analyze the expression of cytokines. The hippocampus tissues were homogenized, and the levels of IL-1β (Elabscience, E-EL-R0012c), IL-6 (Elabscience, E-EL-R0015c), IL-12 (Elabscience, E-EL-R0064c), tumor necrosis factor (TNF)-α (Elabscience, E-EL-R2856c), IL-18 (Elabscience, E-EL-R0567c), colony-stimulating factor (CSF)-1 (Elabscience, E-EL-R0601c), IL-4 (Elabscience, E-EL-R0014c), IL-10 (Elabscience, E-EL-R0016c), transforming growth factor (TGF)-β (Elabscience, E-EL-0162c), and IL-13 (Elabscience, E-EL-R0563c) were determined according to the manufacturers’ instructions.

Xi S., Wang Y., Wu C., Peng W., Zhu Y, & Hu W. (2022). Intestinal Epithelial Cell Exosome Launches IL-1β-Mediated Neuron Injury in Sepsis-Associated Encephalopathy. Frontiers in Cellular and Infection Microbiology, 11, 783049.

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Quantifying Inflammatory Cytokines via ELISA

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Serum, tissue lysates, and cell supernatant were collected to detect the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 using ELISA. TNF-α (E-EL-R2856c, Elabscience, Wuhan, China), IL-1β (E-EL-R0012c, Elabscience), and IL-6 (E-EL-R0015c, Elabscience) kit were used. ELISA was performed according to the manufacturer’s protocol.

Yang L., Ao Y., Li Y., Dai B., Li J., Duan W., Gao W., Zhao Z., Han Z, & Guo R. (2023). Morinda officinalis oligosaccharides mitigate depression-like behaviors in hypertension rats by regulating Mfn2-mediated mitophagy. Journal of Neuroinflammation, 20, 31.

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IL-6 and TNF-α Quantification by ELISA

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ELISA kits were purchased from Elabscience (Wuhan, China), and the level of IL-6 (E-EL-R0015c, Elabscience) and TNF-α (E-EL-R2856c, Elabscience) detection was detected following the instruction provided.

Liu X., Pan B., Wang X., Xu J., Wang X., Song Z., Zhang E., Wang F, & Wang W. (2023). Ischemia/reperfusion-activated ferroptosis in the early stage triggers excessive inflammation to aggregate lung injury in rats. Frontiers in Medicine, 10, 1181286.

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