Heparin, N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride chromogenic substrate, Heparin cofactor II, monosaccharides, Sephadex G-100, and dextran standards were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Cell culture medium components (F-12 medium), trypsin and newborn calf serum (FCS) were obtained from Cultilab (Campinas, SP, Brazil). l -Glutamine, sodium bicarbonate, sodium pyruvate, and phosphate buffered saline (PBS) were purchased from Invitrogen Corporation (Burlington, ON, USA). All other solvents and chemicals were of analytical grade.
Sephadex g 100
Manufactured by Merck Group
Sourced in United States, Germany, China
Sephadex G-100 is a size-exclusion chromatography media used for the separation and purification of molecules based on their size and molecular weight. It is a porous, cross-linked dextran gel that allows smaller molecules to penetrate the pores while larger molecules are excluded, enabling their separation. Sephadex G-100 is commonly used in various applications such as protein purification, desalting, and molecular weight determination.
Automatically generated - may contain errors
Lab products found in correlation
Deae 52 cellulose, by Merck Group (7 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (5 mentions)
Deae cellulose, by Merck Group (4 mentions)
Triton x 100, by Merck Group (4 mentions)
Acrylamide, by Merck Group (3 mentions)
Coomassie brilliant blue, by Merck Group (3 mentions)
Guaiacol, by Merck Group (3 mentions)
Coomassie brilliant blue r 250, by Merck Group (3 mentions)
Ammonium persulfate, by Merck Group (3 mentions)
Deae sephadex, by Merck Group (3 mentions)
D xylose, by Merck Group (3 mentions)
Bis acrylamide, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Galacturonic acid, by Merck Group (2 mentions)
Sephadex g 50, by Merck Group (2 mentions)
Sodium acetate, by Merck Group (2 mentions)
Coomassie brilliant blue g 250, by Merck Group (2 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (2 mentions)
Deae sepharose, by Merck Group (2 mentions)
52 protocols using sephadex g 100
1
Heparin-based Chromogenic Assay
2
Mung Bean Lipoxygenase Purification
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The mung bean seeds were obtained from Sri Venkateswara Agricultural University, Tirupati. Soybean LOX, ETYA, Linoleic acid, Linolenic acid, Arachidonic acid, Sephadex G-100, DE-52, Poly Buffer Exchangers 94 (PBE-94), Phenyl methyl sulfonyl fluoride (PMSF), EDTA, Acrylamide, Bis-Acrylamide, Coomassie brilliant blue, Lauryl sulphate (SDS) and protein size markers were procured from Sigma Chemicals Co (St. Louis, MO, USA). NDGA was a generous gift from department of chemistry, S.V. University. All other chemicals were reagent grade procured from Merck, Mumbai, India.
3
Protein Purification via Chromatography
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Sephadex G-100, DEAE-cellulose and guaiacol were purchased from Sigma-Aldrich Co, St Louis, USA. Standard protein markers were purchased from Merck, Genei, India. Dyes were collected from local textile industry. All chemicals used were of the highest purity available and of the analytical grade.
4
Anticancer and Anticoagulant Properties of Pleurotus eous
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Fresh fruit bodies of Pleurotus eous were sourced from a Mushroom lab, Bio-Centre, Department of Horticulture, Bengaluru, Karnataka, India. The human cervical cancer cell (HeLa) and human lymphocytic leukaemia cell-lines (Jurkat) were procured from the National Centre for Cell Science, India. DMEM medium, fetal bovine serum (FBS), penicillin and streptomycin were purchased from Gibco/Invitrogen (Grand Island, NY, USA). DEAE cellulose 52, Sephadex G-100, T series dextrans, monosaccharide standards, glucose (Glc), galactose (Gal), arabinose (Ara), mannose (Man), fucose (Fuc), ribose (Rib), rhamnose (Rha), xylose (Xyl), glucuronic acid (GluA), galacturonic acid (GalA), sulfur trioxide-pyridine complex (S03-Py), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Bovine serum albumin (BSA), fetal bovine serum (FBS), dimethylformamide (DMF) Coomassie brilliant blue G250, Bovine serum albumin (BSA), Vitamin C (Vc), were obtained from Sigma Aldrich (St. Louis, MO, USA). The DEAE cellulose 52 for the fast flow was obtained from GE Healthcare Life Science (Piscataway, NJ, USA). APTT, PT and TT reagent kit was purchased from Agappe diagnostic Ltd., Cochin. All other chemicals are analytical reagent grade and purchased from Himedia in Mumbai, India and Merck, India.
5
Purification and Sequencing of Endpoint RT-PCR Products
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We purified the endpoint RT-PCR products with Sephadex® G-100 (Sigma-Aldrich, St. Louis, MO, USA) columns by centrifugation at 770×g for 3 min and then prepared the sequencing reactions with the BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems). The sequencing reactions had a final volume of 10 μl containing 1 μl BigDye® Terminator v3.1 Ready Reaction Mix, 1.5 μl 5× Sequencing Buffer, 0.2 μM of either the forward (FS778) or the reverse (cFD2) primer and 3 to 10 ng cDNA. The temperature profiles were as follows: 1 min at 96 °C, followed by 25 cycles of 10 s at 96 °C, 5 s at 50 °C and 4 min at 60 °C. We then purified the sequencing products with Sephadex® G-50 (Sigma-Aldrich) columns by centrifugation at 770×g for 3 min and mixing with 5 μl of HiDi Formamide (Applied Biosystems) and sequenced the prepared cDNA in both directions on an Applied Biosystems 3500 Genetic Analyser (Applied Biosystems). We examined the sequences with the MEGA6 Software [37 (link)] and compared them against the BLAST Nucleotide database [38 (link), 39 ].
6
Purification and Analysis of Peroxidase Enzyme
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2, 2’ Azino bis[3 ethylbenzthiazoline 6 sulfonate] (ABTS), acrylamide, ammonium persulfate, bis-acrylamide, coomassie brilliant blue R-250, and TEMED (N, N, N’, N’- Tetramethyl ethylenediamine), Sephadex G-100, Reactive Blue-4 were purchased from Sigma-Aldrich. DEAE cellulose and Guaiacol were purchased from Merck, India. Protein marker was purchased from Genei, Bangalore. All other chemicals used were of analytical grade.
7
Purification of Antioxidant Compounds from Hen Eggs
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Fertilized hen eggs on days 15 of incubation were obtained from Yinkun Co. (China), which were incubated at 37.8℃ with the relative humidity of 60%. Eggs were automatically turned through with a 90° angle every hour. DEAE Cellulose FF was purchased from GE Healthcare Co. (USA). Sephadex G-100 was purchased from Sigma Co. (USA). The AKTA Purifier Plus chromatographic system (GE Healthcare Co.) was provided by the Hefei University of Technology. The compounds 2,2-diphenyl-1-picrylhydrazyl (abbreviated as DPPH), pyrogallic acid, and 1,10-phenanthroline were purchased from Biyuntian Biotechnology Co. (China). Other chemicals were of analytical grade and provided by the Laboratory of Biotechnology and Food Engineering at Hefei University of Technology.
8
Isolation and Characterization of Choerospondias axillaris Leaf Polysaccharides
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The Choerospondias axillaris leaves were collected from Guangxi Medicinal Botanical Garden (Guangxi, China) and identified by Prof. Yong Tan. Glucose, arabinose, Galactose, galacturonic acid, glucuronic acid, ribose, xylose, Mannose, Rhamnose, Rhamnose (Rha) and fucose were purchased from Borui Sugar Biotechnology Co., Ltd. (Yangzhou, China). DEAE-52 cellulose, Sephadex G-100, Sodium acetate and Trifluoroacetate were purchased from Sigma Chemical Co (Shanghai, China). 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP), nitroblue tetrazolium (NBT), 1,1-diphenyl-2-picrylhydrazyl (DPPH), phenazine methosulphate (PMS), reduced nicotinamide adenine dinucleotide (NADH), ferrozine, [2,2′-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid)] diammonium salt (ABTS) and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were purchased from Aladdin Chemical Co (Shanghai, China). Hydrogen peroxide (30%) water solution, PBS buffer solution (PH 7.2–7.4), 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl and free radical and absolute ethanol (99.5%) were purchased from Guangxi Nanning Chenze Experimental Technology Company (Nanning, China).
9
Enzyme Extraction and Purification Protocol
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Ammonium sulfate ((NH4)2SO4), Rochelle salt (sodium potassium tartrate, C4H4O6KNa·4H2O), sodium hydroxide (NaOH), sodium bisulfite, 3,5-dinitro salicylic acid, carboxymethyl cellulose (CMC) powder, 95%ethanol, Lougle’s iodine solution, o-phosphoric acid (H3PO4), ready-prepared nutrient agar, dialysis bag, Sephadex G-100, and Coomassie Brilliant Blue (CBB) were acquired from Sigma Aldrich (Egypt). All reactions were achieved using sterilized distilled water (dH2O).
10
Purification of Polygalacturonic Acid
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Polygalacturonic acid (PGA) and Sephadex G-100 were purchased from Sigma Chemical Company (St. Louis, MO, USA). Rests of the chemicals were procured either from Merck (Navi Mumbai, India) or S.D. Fine (Mumbai, India) and were used without further purification.
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