Each participant provided a fasting blood sample (fluoride plasma, tubes cooled at +4 °C). FPG was measured photometrically (Glucose 201+ Analyzer, HemoCue, Ångelholm, Sweden) and is presented as plasma equivalents. Serum triglycerides and HDL-cholesterol were measured by colorimetric assays (ABX Pentra400, Horiba Medical, Reichenbach, Germany). The inter-assay CVs were 4.5% and 1.8%, respectively. The total adiponectin concentration was measured using a commercially available ELISA with intra- and inter-assay CVs of 4.9% and 6.7% (BioVendor, Heidelberg, Germany).
Glucose 201 analyzer
Manufactured by HemoCue
Sourced in Sweden
The HemoCue Glucose 201 analyzer is a portable, handheld device that measures glucose levels in a small blood sample. It provides rapid and accurate results, making it a useful tool for healthcare professionals in various settings.
Automatically generated - may contain errors
Lab products found in correlation
Abx pentra 400, by Horiba (2 mentions)
Humalog, by Eli Lilly (1 mentions)
Accu chek, by Roche (1 mentions)
Cobas e411, by Roche (1 mentions)
Td 08028, by Inotiv (1 mentions)
Variant device, by Bio-Rad (1 mentions)
Insulin elisa kit, by Agilent Technologies (1 mentions)
Beckman analyzer 2, by Beckman Coulter (1 mentions)
Accu chek, by Abbott (1 mentions)
Glucose 201 rt, by HemoCue (1 mentions)
Kryptor compact plus device, by Thermo Fisher Scientific (1 mentions)
Brahms copeptin proavp kryptor, by Thermo Fisher Scientific (1 mentions)
16 protocols using glucose 201 analyzer
1
Fasting Blood Biomarkers Analysis
2
Serum Phospholipid Fatty Acid Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The proportions of the most commonly measured FAs in epidemiological studies [11 (link)] were analyzed in serum phospholipids. Fasting serum phospholipids reflect the dietary intake of FAs during the past weeks and are not influenced by recent FA intake [6 (link)]. The details of FA analysis are presented in Additional file 1 . In brief, FAs were extracted from serum samples with tert-butyl methyl ether/methanol, followed by a solid phase separation, hydrolysis and methylation with trimethyl sulfonium hydroxide. The FA methyl esters were separated by their retention time in the gas chromatograph with a 100 m capillary column (HP-88) and detected by flame ionization. The 28 FAs were identified by standard substances and quantified as area percentage of each FA relative to the total area of all detected FAs.
Fasting glucose was measured in full venous blood in mmol/L by on-site photometry (Glucose 201+ Analyzer, HemoCue, Germany); inter-assay coefficients of variation ranged between 1.7% and 6.1%. Serum triglycerides and high-density lipoprotein (HDL)-cholesterol were measured by colorimetric assays (ABX Pentra400, Horiba Medical, Germany). The inter-assay coefficients of variation were 4.5% and 1.8%, respectively. Low-density lipoprotein (LDL)-cholesterol was calculated according to the Friedewald equation [12 (link)].
Fasting glucose was measured in full venous blood in mmol/L by on-site photometry (Glucose 201+ Analyzer, HemoCue, Germany); inter-assay coefficients of variation ranged between 1.7% and 6.1%. Serum triglycerides and high-density lipoprotein (HDL)-cholesterol were measured by colorimetric assays (ABX Pentra400, Horiba Medical, Germany). The inter-assay coefficients of variation were 4.5% and 1.8%, respectively. Low-density lipoprotein (LDL)-cholesterol was calculated according to the Friedewald equation [12 (link)].
3
Glucose and Insulin Tolerance Tests
4
Capillary Blood Glucose and Insulin Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Capillary blood was obtained by finger prick using the Accu-Chek sterile, single-use lancing device (Roche, Germany). Before a finger prick, subjects were encouraged to warm their hand to increase blood flow. To minimize plasma dilution, fingertips were not squeezed to extract blood but were instead gently massaged starting from the base of the hand moving towards the tips.
The first two drops of expressed blood were discarded, and the next drop was used for testing.
Blood glucose was measured using the HemoCue Glucose 201 analyzer (HemoCue AB, Angelholm Sweden). Venous blood samples collected on the test days were centrifuged at 1500 g for 10 minutes at 4 °C, and serum was aliquoted and stored at -80 °C until being analyzed for insulin concentrations later. Serum insulin concentrations were determined using a Cobas e411 (Roche, Hitachi, USA), where the intra-and inter-assay CVs were <5% and <6% respectively.
The first two drops of expressed blood were discarded, and the next drop was used for testing.
Blood glucose was measured using the HemoCue Glucose 201 analyzer (HemoCue AB, Angelholm Sweden). Venous blood samples collected on the test days were centrifuged at 1500 g for 10 minutes at 4 °C, and serum was aliquoted and stored at -80 °C until being analyzed for insulin concentrations later. Serum insulin concentrations were determined using a Cobas e411 (Roche, Hitachi, USA), where the intra-and inter-assay CVs were <5% and <6% respectively.
5
Diabetic Atherosclerosis in Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Streptozotocin (STZ; 50 mg/kg body weight) was administered intraperitoneally daily in 8-week-old male Tg-hCBS Cbs ApoE−/− mice for 5 days to induce HG (blood glucose [Glu] concentration ≥300 mg/dL). At 10 weeks of age, mice were switched to a high-fat diet (21.0% fat, TD 08028; Harlan Teklad) and/or Zn water for 2 weeks (Fig. 1A ). For the blood Glu measurement, study mice were fasted overnight, and blood samples were obtained from the tail vein, collected in HemoCue Glucose 201 Microcuvettes (HemoCue, Brea, CA) and analyzed on a HemoCue Glucose 201 analyzer.
All experiments were conducted in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and with approval from Temple University School of Medicine Institutional Animal Care and Use Committee.
All experiments were conducted in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and with approval from Temple University School of Medicine Institutional Animal Care and Use Committee.
6
Glucose Tolerance Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Participants were asked to fast overnight for at least 10 h. Upon arrival at the research unit, fasting blood samples were collected, and FPG was tested using a point-of-care HemoCue® Glucose 201 analyzer (HemoCue Inc., Lake Forest, CA). Subsequently, another set of blood samples were collected to measure PG after a 2-h 75-g OGTT. Briefly, volunteers were given 75 g of glucose in a 250-mL solution to drink, and 2-h post consumption, PG was measured using the Siemens Dimension RXL chemistry analyzer (Diamond Diagnostics, Holliston, MA). HbA1c levels were determined using a VariantT M device (BioRad, Hercules, CA).
7
Glucose Tolerance Test in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four weeks old male WT and Fst-Tg mice were fasted overnight and anesthetized with an IP injection of sodium pentobarbitone (100 mg/kg). A silastic catheter filled with heparinized saline (20 µ/ml) was inserted into left carotid artery. A bolus of glucose (1g/kg body weight) was injected into the IP cavity. 25 µl of blood was collected at 0, 30, 60, 120, 180, and 240 minutes for plasma glucose using HemoCue Glucose 201 analyzer (Ängelholm, Sweden) analysis.
8
Measurement of Glucose and Hormones in OGTT
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood glucose concentration was measured at OsloMet using HemoCue Glucose 201 Analyzer and Microcuvettes. The Microcuvettes were stored in a refrigerator (4°C) and taken out in room temperature 30 min prior to blood sampling. Insulin and hsCRP were measured in serum before, at 30, 60 and 120 min after the OGTT. Triglycerides and total cholesterol were measured in serum fasted at every visit. Serum was obtained from 8.5 ml serum gel tubes and turned 6-10 times before spin down after 30 min (1300 – 1500 g, 15 min), and kept in a refrigerator (4°C) before it was sent to a routine laboratory (Fürst Medical Laboratory, Oslo, Norway) within 24 h.
Peptide YY (PYY) and glucagon-like peptide 2 (GLP-2) were measured in plasma before and 30, 60, and 120 min after the OGTT. Plasma was obtained from EDTA-tubes, immediately placed on ice and centrifuged within 10 min (1500 g, 4°C, 10 min). EDTA-plasma were stored at −80°C and shipped to a commercial laboratory for analysis (Vitas Analytical Service, Oslo, Norway).
Peptide YY (PYY) and glucagon-like peptide 2 (GLP-2) were measured in plasma before and 30, 60, and 120 min after the OGTT. Plasma was obtained from EDTA-tubes, immediately placed on ice and centrifuged within 10 min (1500 g, 4°C, 10 min). EDTA-plasma were stored at −80°C and shipped to a commercial laboratory for analysis (Vitas Analytical Service, Oslo, Norway).
9
Intranasal Insulin Protocol for Hormonal Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples for the determination of blood glucose levels and circulating concentrations of hormones were obtained before intranasal insulin administration and repeatedly throughout the night. Blood glucose was determined immediately after each blood draw (HemoCue Glucose 201 Analyzer, HemoCue AB, Ångelholm, Schweden). The remaining samples were centrifuged and serum and plasma were frozen at −80°C for later analyses. Insulin concentrations were determined in young and elderly participants (Insulin ELISA Kit, Dako, Glostrup, Denmark). In the young participants, plasma concentrations of total ghrelin (RIA; Linco Research, St. Charles, MO; sensitivity 93 pg/ml, intra-assay and inter-assay CV, 10 and 17.8%) and serum concentrations of leptin (RIA; Linco Research, St. Charles, MO; sensitivity, 0.5 ng/ml, intra-assay and inter-assay CV, 8.3% and 6.2%) were measured at time-points of relevance throughout the night.
10
Plasma Biomarker Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma assays were performed as previously described (31 (link), 36 (link)). Briefly, plasma glucose was measured on a Beckman Analyzer II (Beckman, Fullerton, CA, USA) in rats and on a HemoCue Glucose 201 Analyzer (HemoCue Inc., CA, USA) in mice. Plasma FFA were measured with an enzymatic colorimetric kit (Wako Industries, Neuss, Germany). Radioimmunoassays specific for rat/mouse insulin and C-peptide (Linco, St. Charles, MO, USA) were used to determine their plasma concentrations. All assays were performed in duplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!