W1 spinning disk head

Manufactured by Yokogawa

Sourced in Japan

The W1 spinning disk head is a key component in Yokogawa's line of lab equipment. It is designed to rapidly spin a sample disk for the purpose of sample preparation and analysis. The core function of the W1 is to provide a stable and consistent spinning motion to the sample disk, enabling precise and repeatable measurements.

Automatically generated - may contain errors

Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Matrigel coated 4 well chamber slides, by Merck Group (2 mentions) Nis elements ar software, by Nikon (2 mentions) X100 100ml, by Merck Group (1 mentions) Ti microscope, by Yokogawa (1 mentions)

Close spelling variants of this product

CSU-W1 spinning-disk head (13 citations) W1 spinning disk (9 citations) W1 spinning-disk scan head (5 citations) CSU-W1 spinning-disk confocal head (3 citations) CSU-W1 Sora spinning disk scan head (3 citations)

3 protocols using w1 spinning disk head

hiPSC-CM Immunofluorescence Imaging

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hiPSC-CMs were seeded at 25k/cm2 in Matrigel-coated 4-well chamber slides (Millipore, PEZGS0416) and cultured for 72h in DMEM-based cardiomyocyte culture media. Cells were fixed with 4% paraformaldehyde for 5 min at room temperature and permeabilized with 0.2% Triton X-100 in 1x PBS for 5 min at room temperature. Cells were rinsed 2x with 1x PBS and incubated for 10 min in the dark at room temperature with 1:2000 Hoechst 33342 (Thermo Fisher, H3570). Cells were imaged at 40x using a custom Nikon ECLIPSE Ti spinning disk confocal microscope with a Yokogawa W1 spinning disk head (Yokogawa, CSU-W1), using 405 and 488 nm lasers. Images were captured using Nikon NIS Elements AR software.

Loiben A.M., Chien W.M., Friedman C.E., Chao L.S., Weber G., Goldstein A., Sniadecki N., Murry C.E, & Yang K.C. (2023). Cardiomyocyte apoptosis contributes to contractile dysfunction in stem cell model of MYH7 E848G hypertrophic cardiomyopathy. bioRxiv.

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Immunocytochemistry of hiPSC-Derived Cardiomyocytes

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hiPSC-CMs were seeded at 25 k/cm² in Matrigel-coated 4-well chamber slides (Millipore, PEZGS0416, Burlington, MA, USA) and cultured for 72 h in DMEM-based cardiomyocyte culture media. Cells were fixed with 4% paraformaldehyde for 5 min at room temperature and permeabilized with 0.2% Triton X-100 (Sigma, X100-100ML, St. Louis, MO, USA) in 1× phosphate buffer saline (PBS) for 5 min at room temperature. Cells were rinsed twice with 1× PBS and incubated for 10 min in the dark at room temperature with 1:2000 Hoechst 33342 (Thermo Fisher, H3570, Waltham, MA, USA). Cells were imaged with 40× objective using a custom Nikon ECLIPSE Ti spinning disk confocal microscope with a Yokogawa W1 spinning disk head (Yokogawa, CSU-W1, Tokyo, Japan), using 405 and 488 nm lasers. Images were captured using NIS Elements AR software (Version 5.02.01, Nikon, Tokyo, Japan).

Loiben A.M., Chien W.M., Friedman C.E., Chao L.S., Weber G., Goldstein A., Sniadecki N.J., Murry C.E, & Yang K.C. (2023). Cardiomyocyte Apoptosis Is Associated with Contractile Dysfunction in Stem Cell Model of MYH7 E848G Hypertrophic Cardiomyopathy. International Journal of Molecular Sciences, 24(5), 4909.

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Multilayer Hydrogel Cell Isolation

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Multilayer hydrogels containing layers of HS5-mTagBFP2, HS5-eGFP, and HS5-mCherry cells were formed either by sequential casting of hydrogels between glass slides to form a bullseye pattern (Flow Cytometry Experiments) or by pipetting gel precursor into the patterning devices for sequential casting steps (Imaging Experiments). Composite gels were then sequentially treated with sortases as described above, with each dissolution step proceeding for 1 hr. Released cells were collected and fixed in 4% paraformaldehyde in PBS (10 mins, RT) prior to analysis on a FACSCanto RUO cytometer (BD Bioscience; San Jose, CA). Fixed multilayer gels were also imaged after sequential dissolution steps on a Nikon Ti microscope with a Yokogawa W1 spinning disk head under 10x magnification.

Bretherton R.C., Haack A.J., Kopyeva I., Rahman F., Kern J.D., Bugg D., Theberge A.B., Davis J, & DeForest C.A. (2023). User-controlled 4D biomaterial degradation with substrate-selective sortase transpeptidases for single-cell biology. Advanced materials (Deerfield Beach, Fla.), 35(19), e2209904.

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