Human umbilical vein endothelial cells (HUVECs) (Lonza) were cultured in Endothelial Basal Medium (MCDB 131) without pyruvate (USBiological), and used at a low passage number (1–6) for in vitro assays. Media was supplemented with EGM-2 SingleQuot growth factors (Lonza), 1% GlutaMax (Gibco), and 400μM Uridine (supplemented MCDB 131 media). B16-F10 melanoma cells were cultured in RPMI (Corning), with 10% FBS (Corning), 1% sodium pyruvate (Gibco), 1% Non-essential amino acids (Gibco), 1% GlutaMax (Gibco), 1% antibiotic/antimycotic (Corning), and 0.05mM β-mercaptoethanol (Sigma). Cells were maintained at 37˚C with 5% CO2. HUVECs were treated with: 25nM Antimycin A (Sigma), 250nM Piericidin (Sigma), 1mM methyl pyruvate (MP) (Sigma), and/or indicated doses of L-Aspartic Acid (aspartate) (Sigma), L-aspartic acid dimethyl ester hydrochloride (methyl aspartate) (Sigma), or L-asparagine (asparagine) (Sigma).
L aspartic acid dimethyl ester hydrochloride methyl aspartate
Manufactured by Merck Group
L-aspartic acid dimethyl ester hydrochloride (methyl aspartate) is a chemical compound commonly used as a laboratory reagent. It serves as a precursor for the synthesis of various organic compounds. The product provides the aspartic acid moiety in a protected form, which can be useful for various chemical transformations and applications in organic synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Egm 2 singlequot growth factors, by Lonza (2 mentions)
Antibiotic antimycotic, by Corning (2 mentions)
Piericidin, by Merck Group (2 mentions)
Methyl pyruvate, by Merck Group (2 mentions)
L aspartic acid aspartate, by Merck Group (2 mentions)
L asparagine asparagine, by Merck Group (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Antimycin a, by Merck Group (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
β mercaptoethanol, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Corning (2 mentions)
Huvecs, by Lonza (2 mentions)
Roswell park memorial institute (rpmi), by Corning (2 mentions)
2 protocols using l aspartic acid dimethyl ester hydrochloride methyl aspartate
1
HUVEC Metabolic Regulation Assay
2
HUVEC Metabolic Regulation Assay
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