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The Gr33a1 is a laboratory equipment item used for the analysis and study of Drosophila, or fruit flies. It serves as a core function in the research and development activities at the Bloomington Drosophila Stock Center. The detailed specifications and intended use of this product are not available for an unbiased and factual description.

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2 protocols using gr33a1

1

Drosophila Gustatory Receptor Mutant Lines

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Oregon R, w1118, w1, Gr39ak05106, Gr33aGal4, Gr33a1, UAS-Gr33a, UAS-APPΔCT, UAS-GFP and UAS-APP were obtained from Bloomington Drosophila Stock Center. Gr32aMI04430 was a gift from Dr. Zuoren Wang. Δppk23 and Δppk29 were gifts from Dr. Kristin Scott. UAS-TNT and UAS-TNTin were gifts from Dr. Aike Guo. dfoxOΔ94 was generously provided by Dr. Linda Partridge. Wild type (WT) controls for the mutants (Gr32a, Gr33a, Gr39a, ppk23, ppk29) were from the corresponding background strains.
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2

Drosophila Husbandry and Genotypes

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Flies were raised on standard cornmeal-dextrose diet at 25°C and 50% relative humidity in a 12:12 light:dark cycle. For the diet, yellow cornmeal (57020, Quaker), dextrose (G8270, Sigma), inactive dry yeast (75570, Lynside), propionic acid (402907, Sigma) and tegosept (20–258, Apex chemical and reagent) were used. D. melanogaster flies were red-eyed Canton-S. Other D. melanogaster genotypes were obtained from the Bloomington Drosophila Stock Center: UAS-Kir2.1 (BL91802), Gr64f-Gal4 (BL27883), Gr32a-Gal4 (BL57622), Gr33a1 (BL31427), Ir56d1 (BL81249), Ir76b1 (BL51309), orco1 (BL23129), vas-Cas9 (BL# 51324), w1118 (BL5905). wCS is w1118 backcrossed to Canton-S. D. simulans (w[501] 14021–0251.195) and D. sechellia (14021–0248.25) were obtained from the Drosophila Species Stock Center. ΔGr64a-f flies were generously shared by Dr. Seok Jun Moon. Ir47a1 mutants were generated in the laboratory for this study.
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