Leica dmrb

Manufactured by Leica Microsystems

Sourced in Germany, Denmark

The Leica DMRB is a research-grade inverted microscope designed for a wide range of applications in life science and materials science research. It features a modular design, allowing for customization to meet specific experimental requirements. The DMRB provides high-quality imaging and advanced illumination options to support various microscopy techniques, including brightfield, darkfield, phase contrast, and fluorescence.

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Lab products found in correlation

Bioclear, by Bio-Optica (1 mentions) Moticam 3, by Motic (1 mentions) Flica caspase 3 7 assay kit, by ImmunoChemistry Technologies (1 mentions) Infinite 500, by Tecan (1 mentions) Leica tsc sp2, by Leica Microsystems (1 mentions) Moticam 10 camera, by Motic (1 mentions) Leica m125, by Leica camera (1 mentions)

5 protocols using leica dmrb

Hedgehog Parasitology: Hearts, Lungs, and Livers

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The hearts and lungs of 276 hedgehogs were examined. Hearts were opened with scissors and examined for heartworms under a dissection microscope. Lungs were cut open along the bronchi and bronchioles and microscopically examined for lungworms using a dissection microscope (Leica DMRB, Leica Microsystems A/S, Brønshøj, Denmark). Subsequently, the lungs were flushed several times with tap water into a conical glass and left to sediment for 20 min. Afterwards, the supernatant was removed and the sediment was transferred to glass slides for microscopic identification and enumeration of lungworms (Leica DMRB, Leica Microsystems A/S, Brønshøj, Denmark).
The livers of 94 hedgehogs were macro- and microscopically examined for abscesses, helminths and eggs of C. hepatica, using a dissection microscope (Leica DMRB, Leica Microsystems A/S, Brønshøj, Denmark). The livers were placed on a petri dish and back-illuminated with a strong light source to detect potential internal lesion or cysts, which are invisible from the liver surface. Abscesses were separated from the surrounding liver material, transferred to a microscopic slide, and microscopically examined for protoscolices, including those from Echinococcus multilocularis (Leica M125, Leica Microsystems A/S, Brønshøj, Denmark).

Rasmussen S.L., Hallig J., van Wijk R.E, & Petersen H.H. (2021). An investigation of endoparasites and the determinants of parasite infection in European hedgehogs (Erinaceus europaeus) from Denmark. International Journal for Parasitology: Parasites and Wildlife, 16, 217-227.

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Microscopic Evaluation of Verticillium Leaf Infection

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The third cotton leaves that had no visible wounds were selected and the leaf surfaces were smeared with the conidial suspension of V. dahliae (1–3 × 10⁷ ml). Leaves were collected at 0, 0.5, 1, 3, 5, 8, or 12 hr and stained with trypan blue using the method described by Lipka et al.41 (link). In addition, roots of transgenic line H213 and parent W0 plants at the 2 to 3 leaf stage were inoculated with a conidial suspension of V. dahliae (5 × 10⁶/ml) according to the method described above. Leaves were collected 15 days after inoculation with V. dahliae and then stained with trypan blue. Stained leaf samples were observed under a Leica light microscope (Leica DMRB, Leica Microsystems, Germany) and photographed with a Leica DFC camera (DM2500-3HF-FL, Leica Microsystems, Germany). Leaves without any wounds or visible symptoms of the disease from 10 independent H213 plants were examined.

Zhang Z., Zhao J., Ding L., Zou L., Li Y., Chen G, & Zhang T. (2016). Constitutive expression of a novel antimicrobial protein, Hcm1, confers resistance to both Verticillium and Fusarium wilts in cotton. Scientific Reports, 6, 20773.

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Histological Analysis of EpiIntestinal Samples

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Each EpiIntestinal insert was washed in phosphate buffered saline (PBS 0.1 M, pH 7.4) and fixed for 1 h in buffered 4% paraformaldehyde (PAF). To be sure that the fixative reached immediately all the cells, the PAF was also gently poured on the insert. After fixation, the inserts were washed again in PBS at 4 °C; dehydrated in ethanol series; cleared in Bioclear (Bio-Optica, Milan, Italy); and finally, embedded in paraffin. The samples were cut in 5 µm-thick sections and put on glass slides. Sections were stained with hematoxylin-eosin histological staining or Alcian-Periodic Acid Schiff (PAS) histochemical staining (Bio-Optica, Milan, Italy) and observed through a light microscope Leica DMRB (Leica Microsystems, Wetzlar, Germany) equipped with a Moticam 3+ (Motic Europe, Barcelona, Spain).

Allaria G., De Negri Atanasio G., Filippini T., Robino F., Dondero L., Soggia F., Rispo F., Tardanico F., Ferrando S., Aicardi S., Demori I., Markus J., Cortese K., Zanotti-Russo M, & Grasselli E. (2022). Innovative In Vitro Strategy for Assessing Aluminum Bioavailability in Oral Care Cosmetics. International Journal of Environmental Research and Public Health, 19(15), 9362.

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Quantifying Caspase-3/7 and CTSB Activity

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In vivo caspase-3/7 activity was measured by FAM-fluorochrome inhibitor of caspases (FLICA) Caspase 3&7 Assay Kit (Immunochemistry Technologies, Bloomington, MN, USA) according to the manufacturer's information. Briefly, cells were incubated with the FAM-DEVD-fluoromethylketone (FMK) reagent for 1 h at 37 °C. After washing with PBS, cells were fixed with 0.1% (w/v) formaldehyde solution. In vivo CTSB activity was performed using Magic Red CTSB Activity Kit (Immunochemistry Technologies) according to the manufacturer's protocol. Cells were incubated with Magic Red CTSB Substrate (Immunochemistry Technologies) for 1 h at 37 °C. The cells were pelleted after centrifugation, washed with PBS, and fixed with 0.1% formaldehyde. Fluorescence was measured by a fluorescent microplate reader Infinite 500 (Tecan Group Ltd., Männedorf, Switzerland), or at least 200 cells were counted and indicated as the percentage of positive cells per total cells. Images were obtained by fluorescent microscopy Leica DMRB or confocal microscopy Leica TSC SP2 (Leica Microsystems, Wetzlar, Germany).

Taniguchi M., Ogiso H., Takeuchi T., Kitatani K., Umehara H, & Okazaki T. (2015). Lysosomal ceramide generated by acid sphingomyelinase triggers cytosolic cathepsin B-mediated degradation of X-linked inhibitor of apoptosis protein in natural killer/T lymphoma cell apoptosis. Cell Death & Disease, 6(4), e1717-.

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Olfactory System Analysis Protocol

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One olfactory organ for each specimen was analyzed through a stereomicroscope Leica DMRB (Leica Microsystems, Wetzlar, Germany) equipped with a Moticam 10+ camera (Motic Europe, Barcelona, Spain) to count the olfactory lamellae, to evaluate the OSA, and to measure the olfactory nerve thickness using ImageJ [43 (link)]. As specified, all the measurements were collected after fixation and storage in 70% ethanol.

Aicardi S., Bozzo M., Amaroli A., Gallus L., Risso B., Carlig E., Di Blasi D., Vacchi M., Ghigliotti L, & Ferrando S. (2022). The Arrangement of the Peripheral Olfactory System of Pleuragramma antarcticum: A Well-Exploited Small Sensor, an Aided Water Flow, and a Prominent Effort in Primary Signal Elaboration. Animals : an Open Access Journal from MDPI, 12(5), 663.

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