Verikine hs human interferon alpha all subtype elisa kit

Levels of serum IFN-α were determined in patients with SLE and in HC using VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit (PBL Assay Science, Piscataway Township, NJ, USA) according to the manufacturer’s instructions.

Serum archived at the time of recruitment was stored at −80 °C and thawed immediately prior to testing. Serum levels of IFN-α were measured in duplicate using the VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit (PBL Assay Science, Piscataway, NJ, USA). The lower limit of detection of this kit is 1.95 pg/ml.

IFNα and IFNλ production by 2’3’ cGAMP-treated STING-transduced BJAB cells were measured in triplicate using commercial sandwich ELISA assay sets (IFNα: VeriKine-HS™ Human Interferon Alpha All Subtype ELISA Kit, PBL Assay Science; Piscataway, NJ, and IFNλ: Human IL-29/IL-28B [IFN-lambda 1/3] DuoSet ELISA set, R&D Systems; Minneapolis, MN) according to the manufacturer’s instructions. Standard protein samples were diluted in cell culture media for accurate standard curve construction and calculations. Recombinant IFNs were used as positive controls while cell culture media served as the negative control. Biological duplicate samples from each timepoint were each assayed in technical duplicate. The level of sensitivity for the IFNα and IFN λ assays were 12.5 and 62.5 pg/mL, respectively.