Antibodies used in our experiments included anti-SHH, anti-Gli1, anti-snail1, and anti-GAPDH antibodies (Affinity Biosciences, China). PVDF membrane, BCA Protein Assay Kit, and BeyoECL Moon kit were acquired from Beyotime Biotechnology, China. Cyclopamine was purchased from Selleck Chemicals, USA. Experimental instruments included AU400 automatic biochemical analyzer (OLYMPUS, Japan), BX51T-PHD-J11 microscope (OLYMPUS Company, Japan), image acquisition system CMOS (OLYMPUS Company, Japan), and Image-Pro Plus (Media Cybernetics Company, USA), etc.
Bx51tphd j11 microscope
Manufactured by Olympus
Sourced in Japan
The BX51TPHD-J11 is a high-quality microscope designed for laboratory use. It features phase contrast and brightfield imaging capabilities, providing clear and detailed images for various applications. The microscope is equipped with a trinocular observation tube and a high-resolution camera port, enabling both visual observation and digital image capture. The BX51TPHD-J11 is a versatile instrument suitable for a wide range of microscopy tasks.
Automatically generated - may contain errors
Lab products found in correlation
Image pro plus, by Media Cybernetics (4 mentions)
In situ cell death detection kit, by Roche (3 mentions)
Cyclopamine, by Selleck Chemicals (2 mentions)
Au400 automatic biochemical analyzer, by Olympus (2 mentions)
Anti gli1, by Affinity Biosciences (1 mentions)
Anti snail1, by Affinity Biosciences (1 mentions)
Anti gapdh antibody, by Affinity Biosciences (1 mentions)
Beyoecl moon kit, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Pvdf membrane, by Beyotime (1 mentions)
Jem 1400 plus tem, by JEOL (1 mentions)
Trizol, by Takara Bio (1 mentions)
5 protocols using bx51tphd j11 microscope
1
Hedgehog Signaling Pathway Profiling
2
Apoptosis Detection in Tumor Samples
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Tumor specimens were subjected to a TUNEL assay using the In Situ Cell Death Detection kit (Roche, Basel, Switzerland), according to manufacturer’s instructions for detecting apoptosis. As noted above, the fixed tissues were incubated with 100 μL Proteinase K for 30 min at 37°C. Slides were rinsed twice with PBS. TUNEL reaction mixture was added to the sample at 37°C and incubated for 60 min. Converter-POD solution was added to the sample at 37°C and incubated for 30 min. The results were analyzed under an Olympus BX51TPHD-J11 microscope (Tokyo, Japan). The apoptotic rate was determined as the percentage of TUNEL positive cells to overall tumor cells. The analysis software (Image Pro Plus, Media Cybernetics, USA) was used for image and data acquisition and analysis.
3
Molecular Mechanisms of Rhein-Mediated SHH Signaling
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Reagents and instruments used for this study are as follows: rhein (Rhawn, Shanghai, China); cyclopamine (Selleck Chemicals, Houston, USA); human SHH protein (StemRD Inc, USA); antibodies against TGF-β1 and α-SMA (Servicebio, Wuhan, China); antibodies against SHH, Gli1, Snail, and GAPDH (Affinity Biosciences, Jiangsu, China); PVDF membrane, BCA Protein Assay Kit, BeyoECL Moon kit (Beyotime Biotechnology, Shanghai, China); TRIzol (Takara Bio, Shiga, Japan); primers for qPCR (Servicebio, Wuhan, China); AU400 automatic biochemical analyzer (OLYMPUS, Tokyo, Japan); BX51T-PHD-J11 microscope (OLYMPUS Company, Tokyo, Japan); image acquisition system CMOS (OLYMPUS Company, Tokyo, Japan); Image-Pro Plus (National Institutes of Health, Bethesda, USA); and TEM (JEM1400PLUS, JEOL, Tokyo, Japan).
4
Quantification of Apoptosis in Tumor Samples
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Tumor specimens were subjected to a TUNEL assay using the In Situ Cell Death Detection kit (Roche, Basel, Switzerland), according to manufacturer's instructions for detecting apoptosis. As noted above, the fixed tissues were incubated with 100 μl Proteinase K for 30 min at 37 °C. Slides were rinsed twice with PBS. Fifty microliter TUNEL reaction mixture was added to the sample in a humid and dark atmosphere at 37 °C and incubated for 60 min. Then fifty microliter Converter-POD solution was added to the sample at 37 °C incubated for 30 min. DAB substrate was added to the slides, overlaid with a coverslip and analyzed under light microscope.
The numbers of overall tumor and TUNEL positive cells were quantified in five random sections by a light microscope at magnification of 400 ×. The apoptotic index was determined as the percentage of TUNEL positive cells to overall tumor cells. Slides with DAB-stained were analyzed by an Olympus BX51TPHD-J11 microscope (Tokyo, Japan). The analysis software (Image Pro Plus, Media Cybernetics, USA) was used for image and data acquisition and analysis.
The numbers of overall tumor and TUNEL positive cells were quantified in five random sections by a light microscope at magnification of 400 ×. The apoptotic index was determined as the percentage of TUNEL positive cells to overall tumor cells. Slides with DAB-stained were analyzed by an Olympus BX51TPHD-J11 microscope (Tokyo, Japan). The analysis software (Image Pro Plus, Media Cybernetics, USA) was used for image and data acquisition and analysis.
5
TUNEL Assay for Apoptosis Quantification
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Tumor specimens were subjected to a TUNEL assay using the In Situ Cell Death Detection kit (Roche, Basel, Switzerland), according to manufacturer's instructions for detecting apoptosis. As noted above, the xed tissues were incubated with 100 μl Proteinase K for 30 min at 37°C. Slides were rinsed twice with PBS. Fifty microliter TUNEL reaction mixture was added to the sample in a humid and dark atmosphere at 37℃ and incubated for 60min. Then fty microliter Converter-POD solution was added to the sample at 37℃ incubated for 30 min. DAB substrate was added to the slides, overlaid with a coverslip and analyzed under light microscope.
The numbers of overall tumor and TUNEL positive cells were quanti ed in ve random sections by a light microscope at magni cation of 400X. The apoptotic index was determined as the percentage of TUNEL positive cells to overall tumor cells. Slides with DAB-stained were analyzed by an Olympus BX51TPHD-J11 microscope (Tokyo, Japan). The analysis software (Image Pro Plus, Media Cybernetics, USA) was used for image and data acquisition and analysis.
The numbers of overall tumor and TUNEL positive cells were quanti ed in ve random sections by a light microscope at magni cation of 400X. The apoptotic index was determined as the percentage of TUNEL positive cells to overall tumor cells. Slides with DAB-stained were analyzed by an Olympus BX51TPHD-J11 microscope (Tokyo, Japan). The analysis software (Image Pro Plus, Media Cybernetics, USA) was used for image and data acquisition and analysis.
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