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Lung digestion kit

Manufactured by Miltenyi Biotec
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The Lung Digestion Kit is a laboratory tool designed to facilitate the isolation and dissociation of cells from lung tissue samples. It contains a set of reagents and protocols optimized for this specific application.

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Lab products found in correlation

Gentlemacs dissociator, by Miltenyi Biotec (1 mentions) Anti mouse cd34, by BD (1 mentions) Cd31 antibody, by BD (1 mentions) Moflo cell sorter, by Beckman Coulter (1 mentions)

2 protocols using lung digestion kit

1

Dissociation of Whole Lung Tissue

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Whole lungs were dissociated to single cell suspension by mechanical disruption using the GentleMacs dissociator and Lung Digestion Kit (Miltenyi, Auburn, CA) per manufacturer instructions. Total cells were stained for flow cytometry, as described below.
Ather J.L., Foley K.L., Suratt B.T., Boyson J.E, & Poynter M.E. (2015). Airway epithelial NF-κB activation promotes the ability to overcome inhalational antigen tolerance. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 45(7), 1245-1258.
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2

Isolation of Lung Cell Subsets

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Cell dissociation was achieved by digesting mouse lungs from transgenic mice of α-SMA-EGFP or T1a-EGFP with a commercially available Lung Digestion Kit (Miltenyi Biotech, Auburn, CA). Cells were further stained with anti-mouse CD34 (BD Biosciences) or CD31 antibodies (BD Pharmingen, San Diego, CA). Isotype antibodies were used as controls. SMCs (α-SMA-EGFP), or type I epithelial cells (T1a-EGFP) or hematopoietic cells (CD34 positive) or endothelial cells (CD31 positive) were collected using a MoFlo cell sorter (Beckman Coulter, Fullerton, CA).
Cushing L., Costinean S., Xu W., Jiang Z., Madden L., Kuang P., Huang J., Weisman A., Hata A., Croce C.M, & Lü J. (2015). Disruption of miR-29 Leads to Aberrant Differentiation of Smooth Muscle Cells Selectively Associated with Distal Lung Vasculature. PLoS Genetics, 11(5), e1005238.
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