Cell senescence was determined using a β-galactosidase (β-gal) staining kit (Beyotime, China). UC-MSCs were fixed and stained in the solution at 37 °C for 24 h. Then, cells positive for β-gal activity were observed under a microscope.
β galactosidase β gal staining kit
Manufactured by Beyotime
Sourced in China
The β-galactosidase (β-gal) staining kit is a laboratory tool used to detect the presence and activity of the β-galactosidase enzyme. β-galactosidase is an enzyme that catalyzes the hydrolysis of β-galactosides, such as lactose, into monosaccharides. The kit provides the necessary reagents and protocols to perform β-gal staining, a technique widely used in various biological and biochemical applications.
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3 protocols using β galactosidase β gal staining kit
1
Senescence Detection via β-Gal Staining
2
Senescence Analysis of hBMSCs
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hBMSCs in each group (P3, P8, P8-SHED-CM and P8-hBMSCs-CM) were seeded into 6-well culture plates (5 × 104 cells per well) and cultured for 3 days. Senescent cells were stained by a β-galactosidase (β-gal) staining kit (Beyotime, China) according to the manufacturer’s instructions. The β-gal-positive cells were scanned by microscopy, and 6 fields were taken to calculate the proportion of senescent cells in each group.
3
Senescence Detection via β-gal Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell senescence was determined using a β-galactosidase (β-gal) staining kit (Beyotime, China). UC-MSCs were fixed and stained with the solution at 37 °C for 24 h. Then, cells positive for β-gal activity were observed under a microscope.
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