Il 1b af 401 na

Manufactured by R&D Systems

IL-1b (AF-401-NA) is a recombinant human interleukin-1 beta (IL-1β) protein. IL-1β is a pro-inflammatory cytokine that plays a key role in the immune response and inflammatory processes.

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Lab products found in correlation

Ascorbate, by Merck Group (1 mentions) Citrulline, by Merck Group (1 mentions) β actin 13e5, by Cell Signaling Technology (1 mentions) Recombinant mouse ifn γ 485 mi cf, by R&D Systems (1 mentions) Dimethyloxalylglycine dmog, by Cayman Chemical (1 mentions) Hif 1α nb100 479, by Novus Biologicals (1 mentions) S nitroso n acetylpenicillamine snap, by Merck Group (1 mentions) Emricasan, by Selleck Chemicals (1 mentions) Tumor necrosis factor (tnf), by Genentech (1 mentions) Ciap1 2, by R&D Systems (1 mentions) Gne684, by Genentech (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Caspase 1, by Genentech (1 mentions) Lc3b l7543, by Merck Group (1 mentions) Ab25630, by Abcam (1 mentions) Lyso tracker red c1046, by Beyotime (1 mentions) Omnipaque 350, by Merck Group (1 mentions) Hk 2 cell, by American Type Culture Collection (1 mentions) Ab108980, by Abcam (1 mentions) 3 ma hy 19312, by MedChemExpress (1 mentions)

Spelling variants of this product

AF-401-NA (81 citations) IL-1b (31 citations) AF-401 (15 citations)

3 protocols using il 1b af 401 na

Mouse Interferon-gamma Signaling Assay

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Recombinant mouse IFN-γ (485 MI/CF) was obtained from R&D systems. 1400W, and Dimethyloxalylglycine (DMOG) were obtained from Cayman Chemical. Pam3CysK4 (PAM) was obtained from EMC Microcollections. Ascorbate, citrulline, and S-Nitroso-N-acetylpenicillamine (SNAP) were obtained from Sigma-Aldrich. The following primary antibodies were used: HIF-1α (NB100-479, Novus Biologicals), IL-1b (AF-401-NA, R&D systems), and the following Cell Signaling Technology antibodies: HIF-1α (D2U3T), RelA (D14E12), RelB (C1E4), NF-kB1 (D4P4D), IkBa (L35A5), α/β-Tubulin (2148), Histone H3 (D1H2), and β-actin (13E5).

Braverman J, & Stanley S.A. (2017). Nitric oxide modulates macrophage responses to M. tuberculosis infection through activation of HIF-1α and repression of NF-kB. Journal of immunology (Baltimore, Md. : 1950), 199(5), 1805-1816.

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Comprehensive Cell Death Pathway Analysis

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Human recombinant TNF, BV6, GNE684, and GSK583 were all synthesized at Genentech. Emricasan was purchased from Selleck Chemicals (S7775), zVAD from ABclonal and LPS from InvivoGen (tlrl-3peLPS). The primary antibodies used were directed against: RIP1 (610459, BD Biosciences), pRIP1 S166 (#31122, Cell Signaling Technology (CST)), RIP3 (#15828, CST), pRIP3 (#91702, CST), MLKL (#MABc604, Millipore), pMLKL (#37333, CST), FADD (#05-486, Millipore), RIP2 (#22763, Santa Cruz), XIAP (M044-3, MBL, 66800-1-Ig, Proteintech), c-IAP2 (Genentech), c-IAP1/2 (#3400, R&D), caspase-1(Genentech), caspase-3 (#9661, 9662, CST), caspase-7(#9491, CST), caspase-8 (#8592, 9429, 4927, CST), caspase-11(#14340, CST), GSDMD (#50928, CST), IL-1b (AF-401-NA, R&D), JNK(#9252, CST), pJNK (#4668, CST), p65(#8242, CST), p-p65(#3033, CST), IkBa (#9242, CST), p-IkBa (#2859, CST), p38 (#9212, CST), p-p38 (#9211, CST), ERK (#4695, CST), p-ERK (#4370, CST), actin (A3853, Sigma), GapDH (#2118, CST), HSP90 (#4877, CST).

Witt A., Goncharov T., Lee Y.M., Kist M., Dohse M., Eastham J., Dugger D., Newton K., Webster J.D, & Vucic D. (2023). XIAP deletion sensitizes mice to TNF-induced and RIP1-mediated death. Cell Death & Disease, 14(4), 262.

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Investigating Hypoxia-Induced Kidney Cell Response

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Human renal proximal tubular cell line (HK-2 cell) was gained from American Type Culture Collection (ATCC®, CRL-2190). We obtained iohexol from GE Healthcare (Omnipaque 350), mannitol (M9546) from SigmaAldrich, MCC950 (HY-12815), roxadustat (HY-13426) and 3-MA (HY-19312) from MedChemExpress. We obtained antibodies targeting NLRP3 (15101), GAPDH (5174), and cleaved CASP3 (9664) from Cell Signal Technology; CASP1 (sc-56036), BECN1 (sc-48341), and BNIP3 (sc-56167) from Santa Cruz Biotechnology; IL1B (AF-401-NA) from R&D Systems; LC3B (L7543) from Sigma-Aldrich; HIF1A (NB100-105) from Novas Biologicals; VDAC/voltage-dependent anion channel (ab14734), LAMP1/lysosomal-associated membrane protein 1 (ab25630), EGLN2/PHD1/egl-9 family hypoxia-inducible factor 2 (ab108980) from Abcam; TUBA from (AF0001) from Beyotime. We obtained fluorescence secondary antibodies from Abcam: Donkey anti-rabbit IgG (Alexa Fluor® 555, ab150074; Alexa Fluor® 488, ab150073), donkey anti-mouse IgG (Alexa Fluor® 488, ab150105; Alexa Fluor® 555, ab150110). We obtained MitoTracker Red (M7512), and MitoTracker Green (M7514) from ThermoFisher Scientific, and LysoTracker Red (C1046) from Beyotime. The sequences of small interfering RNA (siRNA) were as follows: BNIP3 siRNA 5′- CGUUCCAGCCUCGGUUUCUAUUUAU-3′.

Lin Q., Li S., Jiang N., Jin H., Shao X., Zhu X., Wu J., Zhang M., Zhang Z., Shen J., Zhou W., Gu L., Lu R, & Ni Z. (2020). Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy. Autophagy, 17(10), 2975-2990.

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