CXCR4 expression was analyzed by flow cytometry as previously described [23 (link)]. Breifly, the CXCR4 protein was labeled by mouse anti-CXCR4 primary antibody (Santa Cruz Biotech, TX) in 1:20 dilution to a 200 μL final volume of cells (1 × 105 cells/mL). Antibodies used for flow cytometry included anti-mouse-FITC (BD Biosciences, San Jose, CA), and the samples were latter analyzed using a FACS Calibur/LSRII flow cytometer (Becton-Dickinson, Franklin Lakes, NJ). Data were analyzed using BD FACSDiva software. Gates were set according to isotype controls.
Mouse anti cxcr4 primary antibody
Manufactured by Santa Cruz Biotechnology
The Mouse anti-CXCR4 primary antibody is a laboratory tool designed for the detection and study of the CXCR4 protein, which is a chemokine receptor involved in various cellular processes. This antibody is specific to the CXCR4 protein and can be used in techniques such as Western blotting, immunoprecipitation, and flow cytometry to identify and quantify the presence of CXCR4 in biological samples.
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Lab products found in correlation
3 protocols using mouse anti cxcr4 primary antibody
1
CXCR4 Expression Analysis by Flow Cytometry
2
Cell Surface Receptor Labeling
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Cells were detached using PBS containing 10 mM EDTA and labeled with mouse anti-CXCR4 primary antibody (#4G10, Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-HRH1 monoclonal antibody provided by Dr. Hosun Son (GPCR Therapeutics, Seoul, Korea), or rabbit anti-HA monoclonal antibody (#3724, Cell Signaling Technology). An APC-conjugated goat anti-mouse IgG antibody (R&D Systems, Abingdon, UK) was used to label CXCR4 and HRH1, and goat anti-rabbit IgG-Alexa Fluor 488 antibody (#A32731, Invitrogen) was used to label HA tag. Cells were analyzed with a FACS Canto II (BD Biosciences, San Jose, CA).
3
CXCR4 Expression Analysis in MCF-7 Cells
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