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Anti foxm1 sc 502

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Anti-FOXM1 (sc-502) is a primary antibody that recognizes the FOXM1 (Forkhead Box M1) protein. FOXM1 is a transcription factor that plays a role in cell cycle regulation and proliferation.

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Lab products found in correlation

Anti met sc 161, by Santa Cruz Biotechnology (2 mentions) Anti phosphorylated akt ser472, by Cell Signaling Technology (2 mentions) Quantity one analysis software version 4, by Bio-Rad (2 mentions) Anti akt, by Cell Signaling Technology (2 mentions) Anti stat3, by Cell Signaling Technology (2 mentions) Anti phosphorylated stat3 tyr705, by Cell Signaling Technology (2 mentions) Anti gapdh antibody, by Santa Cruz Biotechnology (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Cycloheximide c4859, by Merck Group (1 mentions) Anti rabbit hrp a 6154, by Merck Group (1 mentions) Anti β actin a2066, by Merck Group (1 mentions) Anti mouse hrp a4416, by Merck Group (1 mentions) Anti o linked n acetylglucosamine antibody rl2 ma1 072, by Thermo Fisher Scientific (1 mentions) Anti β tubulin, by Santa Cruz Biotechnology (1 mentions) Anti caspase 7, by Cell Signaling Technology (1 mentions) Anti caspase 9, by Cell Signaling Technology (1 mentions) P0260, by Agilent Technologies (1 mentions) P0217, by Agilent Technologies (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti ezh2, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

FOXM1 (56 citations) Anti-FOXM1 (33 citations) Sc-502 (9 citations) FOXM1 (sc-502) (2 citations)

5 protocols using anti foxm1 sc 502

1

Investigating O-GlcNAcylation Regulatory Mechanisms

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All primary and secondary antibodies for immunoblotting were used at 1:1000 and 1:2000 dilutions, respectively. The following reagents were used for the experiments in this study: anti-O-linked-N-acetylglucosamine antibody (RL2) (MA1-072) and anti-OGT (PA5-22071) were from Thermo Fisher Scientific; anti-b-actin (A2066), anti-rabbit-HRP (A6154), and anti-mouse-HRP (A4416) were from Sigma-Aldrich; anti-p21 (2947S), anti-p27 (3686S), and anti-Skp2 (4358S) were from Cell Signaling; and anti-FoxM1 (sc-502) was from Santa Cruz. TMG (SML-0244), OSMI-1 (SML-162), and cycloheximide (C4859) were purchased from Sigma-Aldrich. siRNAs against OGT #7 (SI02665131) and #8 (SI04713604) were purchased from Qiagen, and siRNA #1 (SI6094) and siCtrl siRNA (4390843) were purchased from Ambion. A FoxM1 overexpression plasmid (sc-128214) and empty vector control plasmid were purchased from OriGene. Transfection of plasmids was performed using Mirus TransIT X2 transfection reagent, and siRNAs were transfected using Lipofectamine RNAiMax (Thermo Fisher).
de Queiroz R.M., Moon S.H, & Prives C. (2022). O-GlcNAc transferase regulates p21 protein levels and cell proliferation through the FoxM1–Skp2 axis in a p53-independent manner. The Journal of Biological Chemistry, 298(9), 102289.
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2

Quantitative Western Blot Analysis

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Standard Western blot analysis was carried out using primary anti-FOXM1 (sc-502; Santa Cruz Biotechnology), anti-Met (sc-161; Santa Cruz Biotechnology), anti-phosphorylated Met (Tyr1234/Tyr1235 [#8218]; Cell Signaling Technology), anti-AKT (#9272; Cell Signaling Technology), anti-phosphorylated AKT (Ser472 [#9271]; Cell Signaling Technology), anti-p44/42 ERK1/2 (#9102; Cell Signaling Technology), anti-phosphorylated p44/42 ERK1/2 (Thr202/Tyr204 [#8201]; Cell Signaling Technology), anti-STAT3 (#9132; Cell Signaling Technology), and anti-phosphorylated STAT3 (Tyr705 [#9135]; Cell Signaling Technology) antibodies. Anti-mouse (Cell Signaling Technology) and anti-rabbit (Santa Cruz Biotechnology) antibodies were used as secondary antibodies. Equal protein-sample loading was monitored using an anti-GAPDH antibody (Santa Cruz Biotechnology). The bands were quantified using Quantity One analysis software, Version 4.6 (Bio-Rad) and the results of fold changes were expressed as numbers in italic font under individual blot.
Cui J., Xia T., Xie D., Gao Y., Jia Z., Wei D., Wang L., Huang S., Quan M, & Xie K. (2016). HGF/Met and FOXM1 Form a Positive Feedback Loop and Render Pancreatic Cancer Cells Resistance to Met Inhibition and Aggressive Phenotypes. Oncogene, 35(36), 4708-4718.
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3

Quantitative Western Blot Analysis

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Standard Western blot analysis was carried out using primary anti-FOXM1 (sc-502; Santa Cruz Biotechnology), anti-Met (sc-161; Santa Cruz Biotechnology), anti-phosphorylated Met (Tyr1234/Tyr1235 [#8218]; Cell Signaling Technology), anti-AKT (#9272; Cell Signaling Technology), anti-phosphorylated AKT (Ser472 [#9271]; Cell Signaling Technology), anti-p44/42 ERK1/2 (#9102; Cell Signaling Technology), anti-phosphorylated p44/42 ERK1/2 (Thr202/Tyr204 [#8201]; Cell Signaling Technology), anti-STAT3 (#9132; Cell Signaling Technology), and anti-phosphorylated STAT3 (Tyr705 [#9135]; Cell Signaling Technology) antibodies. Anti-mouse (Cell Signaling Technology) and anti-rabbit (Santa Cruz Biotechnology) antibodies were used as secondary antibodies. Equal protein-sample loading was monitored using an anti-GAPDH antibody (Santa Cruz Biotechnology). The bands were quantified using Quantity One analysis software, Version 4.6 (Bio-Rad) and the results of fold changes were expressed as numbers in italic font under individual blot.
Cui J., Xia T., Xie D., Gao Y., Jia Z., Wei D., Wang L., Huang S., Quan M, & Xie K. (2016). HGF/Met and FOXM1 Form a Positive Feedback Loop and Render Pancreatic Cancer Cells Resistance to Met Inhibition and Aggressive Phenotypes. Oncogene, 35(36), 4708-4718.
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4

Protein Expression Analysis via Western Blot

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Cells were harvested with lysis buffer [0.125 m Tris, pH 6.8 at 22°C containing 1% NP-40 (v/v), 2 mM ethylenediamine tetraacetic acid (EDTA), 2 mM N-ethylmaleimide, 2 mM phenylmethanesulphonyl fluoride (PMSF), 1 mM sodium orthovanadate and 0.1 µm sodium okadate] and centrifuged at 4°C for 10 min. Protein concentration was determined by detergent-compatible (DC) protein assay (Bio-Rad). Twenty micrograms of protein were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride membrane and hybridized with the following anti-bodies: anti-FOXM1 (sc-502, Santa Cruz Biotechnology, CA, USA), anti-Caspase 9 (9502, Cell Signaling Technology, MA, USA), anti-KIF2C (WH0011004M1, Sigma), anti-Caspase 7 (9492, Cell Signaling) and anti-β-tubulin (sc-9104, Santa Cruz Biotechnology).
Zhao F., Siu M.K., Jiang L., Tam K.F., Ngan H.Y., Le X.F., Wong O.G., Wong E.S., Gomes A.R., Bella L., Khongkow P., Lam E.W, & Cheung A.N. (2014). Overexpression of Forkhead Box Protein M1 (FOXM1) in Ovarian Cancer Correlates with Poor Patient Survival and Contributes to Paclitaxel Resistance. PLoS ONE, 9(11), e113478.
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5

OTSSP167 Preparation and Antibodies

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OTSSP167 (Biorbyt) was dissolved in DMSO and stored at −20°C. For in vivo studies, OTSSP167 was dissolved in 0.5% methylcellulose (Sigma-Aldrich) and stored at −20°C. The following antibodies were used: anti-FOXM1 (SC-502, Santa Cruz), anti-EZH2 (#4905, Cell Signaling Technology) anti-MELK (GTX111958, GeneTex and 2274S, Cell Signaling Technology), anti-α-tubulin (T6074, Sigma), anti-GAPDH (2118, Cell Signaling Technology), anti-rabbit-HRP (P0217, Agilent) and anti-mouse HRP (P0260, Agilent).
Muller J., Bolomsky A., Dubois S., Duray E., Stangelberger K., Plougonven E., Lejeune M., Léonard A., Marty C., Hempel U., Baron F., Beguin Y., Cohen-Solal M., Ludwig H., Heusschen R, & Caers J. (2018). Maternal embryonic leucine zipper kinase inhibitor OTSSP167 has preclinical activity in multiple myeloma bone disease. Haematologica, 103(8), 1359-1368.
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